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Imported soybean seed is widely used in Indonesia especially as the main ingredient for tofu and soybean cake (tempeh). Genistein, an isoflavone in soybean seed, has an estrogenic activity and act as a strong antioxidant. Besides quantifying genistein in the local and imported soybean seeds, this re...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/10042 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
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id-itb.:100422009-04-17T14:55:18Z#TITLE_ALTERNATIVE# DWIJAYANTI (NIM 10704060), ASRI Indonesia Final Project INSTITUT TEKNOLOGI BANDUNG https://digilib.itb.ac.id/gdl/view/10042 Imported soybean seed is widely used in Indonesia especially as the main ingredient for tofu and soybean cake (tempeh). Genistein, an isoflavone in soybean seed, has an estrogenic activity and act as a strong antioxidant. Besides quantifying genistein in the local and imported soybean seeds, this research was also aimed to find a fast and simple quantitative method. Soybean seed crude drugs were extracted by reflux using methanol. Some parts of the crude drug were delipidated using n-hexane before extracted. Genistein was determined using thin layer densitometry and high performance liquid chromatography (HPLC). Quantity of genistein in local and imported soybean seeds determined by thin layer densitometer were 19.51+-4.59 ug/g and 21.69+-3.98 ug/g, respectively. While the quantity obtained by HPLC were 19.99+-3.79 ug/g and 21.12+-3.19 ug/g, respectively. Both methods showed no significant difference in probability of 0.05. text |
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Institut Teknologi Bandung |
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Institut Teknologi Bandung Library |
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Asia |
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Indonesia Indonesia |
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Institut Teknologi Bandung |
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Indonesia |
| description |
Imported soybean seed is widely used in Indonesia especially as the main ingredient for tofu and soybean cake (tempeh). Genistein, an isoflavone in soybean seed, has an estrogenic activity and act as a strong antioxidant. Besides quantifying genistein in the local and imported soybean seeds, this research was also aimed to find a fast and simple quantitative method. Soybean seed crude drugs were extracted by reflux using methanol. Some parts of the crude drug were delipidated using n-hexane before extracted. Genistein was determined using thin layer densitometry and high performance liquid chromatography (HPLC). Quantity of genistein in local and imported soybean seeds determined by thin layer densitometer were 19.51+-4.59 ug/g and 21.69+-3.98 ug/g, respectively. While the quantity obtained by HPLC were 19.99+-3.79 ug/g and 21.12+-3.19 ug/g, respectively. Both methods showed no significant difference in probability of 0.05. |
| format |
Final Project |
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DWIJAYANTI (NIM 10704060), ASRI |
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DWIJAYANTI (NIM 10704060), ASRI #TITLE_ALTERNATIVE# |
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DWIJAYANTI (NIM 10704060), ASRI |
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DWIJAYANTI (NIM 10704060), ASRI |
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| url |
https://digilib.itb.ac.id/gdl/view/10042 |
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1820664854078291968 |