COLORIMETRICAL ANALYSIS DEVELOPMENT OF QUERCETIN IN FOOD SUPPLEMENT

• Main Author: R. T. MANGINDAAN (NIM 10704070), GRACE
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/10504
• Institution: Institut Teknologi Bandung

Quercetin is a natural antioxidant which is presented in some vegetables and fruits. Quercetin is usually used as food supplement and combined with other antioxidants such as ascorbic acid or other flavonoids. Quercetin determination method is well known mostly for plant materials. This study was to develop quercetin determination method in the presence of ascorbic acid, hesperidin, and calcium in food supplement using colorimetric method. Quercetin was oxidized by N-Bromosuccinimide (NBS) in the presence of phenol resulting colored product which the intensity was equivalent to quercetin amount. Condition of reaction was optimized to the presence of hesperidin, ascorbic acid and calcium. The optimum wavelength for quantification were at 509 nm, and measured at minute two after NBS added. This method had good result when was used with standard addition method and the recovery was 95-110%.