ISOLASI BEBERAPA SENYAWA AKTIF SELAIN ASAM BONGKREK YANG DIPRODUKSI OLEH PSEUDOMONAS COCOVENENANS DAN PENGARUHNYA TERHADAP RESPIRASI

• Main Author: Indrayati Anggraeni, Nenden
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/1061
• Institution: Institut Teknologi Bandung

Pseudomonas cocovenenans is a bacteria that is interesting to be observed in biochemistry, physiology, pharmacology, medicine and especially in food science. From 1880 up to now thousands of people in Banyumas and Kediri died in eases of bongkrec poisoning. The bongkrec bacteria produce poisonous compounds in the medium of tempe bongkrec and especially in the partly defatted coconut, in other words,tempe made from pressed coconut shred can be poisoned by this bacteria. Pseudomonas cocovenenans can be introduced as a contamination before or during fermentation . Under the conditions of the manufacturing process, these bacteria are able to produce toxins from the coconut substrat. These include the colourless bongkrec acid, for which the LD 50 for mice after injection is 1,4 mg per kg bodyweight and a toxoflavin ( mp 171 °C), for which the LD 50 for mice after injection is 1,7 mg per kg of body weight. A crude ethanolic extract of pressed coconut shred fermented with Pseudomonas cocovenenans, was found to inhibit the respiration of bovine heart homogenat ( p > 0,05 ). The aim of this research was the isolation and purification of some compounds with antibiotic activity which are produced by Pseudomonas cocovenenans strain X 128 liophylized in Bogor ( BCC 2184 ). Three toxoflavin have been successfully isolated and purified using the method described by Soedigdo with some improvements, while thin layer chromatography was used for the identification of the three coumpounds. The antibiotic activities towards Escherichia coli and Aspergillus niger were determined with the disk and dilution methods. Three pure compounds were found . The cha - racteristics of the three compounds which have antibi - otic activity are : The factor of retention of the first compound in chloroform : methanol 1 : 1 was about 0,75. It showed white fluorescence and a inciting point of 2160 C. The ultra violet spectroscopy showed two maxima at 268 nm and 360 nm. The infra red spectra showed the following peaks ; 2800, 2900, 1530, 1550, 1460, 1120, 720 cm-l. The second compound showed a factor of retention in chloroform : methanol 1 : 1, of about 0,65, it showed blue - black fluorescence and yellow needle cristals , melting point 205 ° C.In the ultra violet spectrum showed two maxima at 258 nm and 202 nm. The infra red characteristic are ; 700,740, 850, 880,930, 980, 1020,1080, 1120, 1170, 1220, 1260, 1310, 1320, 1460, 1490,1530,1560, 1600,1680, 1770, 2580, 3050, 3500 cm-l. The factor of retention of the third coumpound in chloroform: methanol 1:1 was about 0,60. It showed blue black fluorescence, cream coloured cristals, melting point 181°C. In the ultra violet spectrum it showed two maxima at 328 nm and 202 nm. The antibiotic activity of the white cristals was greater than that of the yellow cristals and the cream cristals.