Isolation, Purification, and Characterization of Thermostable Lipase from Thermophilic Bacteria Local Isolate

• Main Author: RESDIA MEGASARI (NIM 10505082), CITRA
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/11949
• Institution: Institut Teknologi Bandung

Lipase (E.C 3.1.1.3, triacylglycerol hydrolase) is an important enzyme in biotechnological industries since a lot of applications in food, dairy, detergent, pulp, and pharmaceutical industries. Thermophilic microorganism, Geobacillus vulgivagus dYTae-14, isolated from hot spring in Indonesia, showed an extracellular lipase activity on lipid substrates at high temperature. On olive oil (1.5%, w/v) as a sole carbon source, the isolate dYTae-14 grew very rapidly at 70oC and showed maximum lipase activity at late exponential phase, the activity slowly decrease up to stationary phase. The excreted lipase of dYTae-14 was partially purified by ammonium sulfate fractionation to give 4 fractions, 0-30%, 30-80%, 80-100%, and 100% fractions. Fraction of 0-30% showed the highest activity by using para-nitrophenyl palmitate as substrate. The partial purified enzyme showed optimal activity at 65oC and pH 8. <br /> <br />