A3290G Mutation of mtDNA Recombinant In Type II Diabetes Mellitus Patient

• Main Author: HANDAYANI (NIM 10505004), DEWI
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/11991
• Institution: Institut Teknologi Bandung

Diabetes mellitus type II is a desease related to mitochondria and characterized due to reduced insulin sensitivity and maternally inherited. PCR Allele Spesific Alignement (PASA) used to determined diabetes mellitus Type II patient, and giving a positive result for A3243G mutation. This mutation cannot be proved by direct sequencing because mutant DNA copy and wild type DNA copy always in the same part, a condition known as heteroplasmy. The aim of this research is to determined diabetes mellitus Type II patient's mtDNA secuence using DNA recombinant technics. Stage of the research are PASA, cloning, screening and sequencing. PCR amplification gived 0,19 kb DNA fragment. While cloning stage resulting four white colonies that resulting 0,19 kb DNA fragment at screening stage. Sequencing result is an electrophoregram for 720 pb. Homology analysis using rCRS as standard, showed A3290G mutation, mutation that never published before.