Transformation of Saccharomyces cerevisiae with YEp-Secretex-BGL1 Plasmid Containing Trichoderma reesei B-Glucosidase Gen

• Main Author: SINAY (NIM 10505078), MARCELL
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/12353
• Institution: Institut Teknologi Bandung

Bioethanol produced from cellulose-based material is a promising alternative biofuel. Production of biofuel from cellulose involves two steps, namely conversion of cellulose by enzyme or chemical treatment, and then followed by fermentation of glucose into ethanol by yeast or bacterium. Saccharomyces cerevisiae can be transformed into cellulose utilizing yeast by introduction of genes encoding cellulases system. B-Glukosidase (BGL) is one of cellulase multienzyme system. It hydrolyzes B-D-(1,4) glycosidic bond of cellobiose to produce glucose. In this study, Eschericia coli and S. cerevisiae were transformed with an expression vector YEp-Secretex containing gene encoding BGL1 (YEp-BGL1). Recombinant plasmids were isolated and were confirmed by restriction analysis using XbaI and NdeI. Culture supernatant of S. cerevisiae harboring YEp-BGL1 showed B-glukosidase activity at temperature of 50oC and pH 7.0.