PREPARATION AND CHARACTERIZATION OF POLY(METHYL METHACRYLATE)-BENTONITE MEMBRANE TO CONCENTRATE CRUDE EXTRACT OF a-AMYLASE

• Main Author: ARYANA (NIM: 20508011), NURHANI
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/12484
• Institution: Institut Teknologi Bandung

a-Amylase is an enzyme which catalyzes the hydrolysis reaction of a-1,4 glycosidic bonds in starch molecule to dextrin and oligosaccharides. Nowadays, a-amylase is used in many applications, such as detergent, textile and paper industry, especially food industry. Food industry spends billions each year to produce the a-amylase, as a consequence, food price become more expensive. The goal of this research was to prepare poly(methyl methacrylate)-bentonite membranes, then the obtained membranes were used to concentrate crude extract of a-amylase. PMMA-bentonite membranes were prepared by mixing a commercial poly(methyl methacrylate) (PMMA) polymer with bentonite clay in dimethyl formamide solvent, then followed by membrane casting by phase inversion. PMMA-bentonite membranes were made from PMMA as the organic materials and bentonite clay as the inorganic materials.