APPLICATION OF THE NANOBIOSILICA FROM MARINE MICROALGAE THALASSIOSIRA SP. AS A MATRIX FOR GEL FILTRATION COLOUMN
Based on our previous research, biomass of marine microalgae Thallasiosira sp. has been <br /> <br /> <br /> <br /> <br /> used as feedstock for biodiesel production and produces waste of debris of cell walls <br /> <br /> <br /> <br /&g...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/19632 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Based on our previous research, biomass of marine microalgae Thallasiosira sp. has been <br />
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used as feedstock for biodiesel production and produces waste of debris of cell walls <br />
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containing macroporous silica (50 nmm). These nanobiosilica may be used as a matrix <br />
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for gel filtration coloumn. In this present research, nanobiosilica of Thalassiosira sp. was <br />
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studied as a matrix for separation coloumn. To obtain nanobiosilica of Thalassiosira sp., first <br />
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lipid had to be extracted from the cell followed by acid treatment and calcination at 600oC <br />
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with heat rate of 3oC/min for 6 hour. Surface topology and chemical composition of the <br />
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nanobiosilica was characterized by scanning electron microscopy-energy dispersive <br />
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spectroscopy (SEM-EDS). Nanobiosilica of Thalassiosira sp. was applied for matrices of gel <br />
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filtration coloumn to separate mixtures of BSA and lisozyme. The results showed that one <br />
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nanobiosilica was obtained from 100 g wet biomass. The topology of white nanobiosilica of <br />
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Thalassiosira sp. has cylinder in shape with porous size of 80400 nm distributed randomly <br />
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in the surface. The composition of SiO2 of Thalassiosira sp. cells before and after acid <br />
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treatment was 10.8 and 93.79%, respectively. Separation of BSA and lisozim mixtures by <br />
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chromatography coloumn based on a nanobiosilica matrix produced two protein fraction at <br />
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nos. 1517 and 2021. By SDS-PAGE analysis, a protein band of 66 kDa of fraction no. <br />
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1517 was identified as BSA, but no protein band of fraction no. 2021 was observed due to <br />
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a little amount of protein. Thus, a nanobiosilica of Thalassiosira sp. matrix might be applied <br />
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for protein separation |
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