#TITLE_ALTERNATIVE#

• Main Author: NUR IMANIARTI (10712090), DESTRIA
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/21663
• Institution: Institut Teknologi Bandung

Wound can heal naturally through four important stages that are hemostasis, inflammation, <br /> <br /> proliferation, and remodeling. However, infection from bacteria and excess exudate fluid <br /> <br /> can impede the process. Nowadays, the development of wound dressing leads to wound <br /> <br /> dressing that can accelerate wound healing and to promote proliferation of fibroblast. In <br /> <br /> this research, nanofiber formulas were developed using a mixture of chitosan-gelatin and <br /> <br /> chitosan-polyvinyl alcohol for encapsulating propolis as the active agent. The aim of this <br /> <br /> study is to developed mixture hydrogen formula of PVA/chitosan and gelatin/chitosan with <br /> <br /> optimization of polymer concentration and flow rate that can form nanofiber using <br /> <br /> electrospinning which has uniform size, high porosity, biodegradable and strong antioxidant <br /> <br /> activity. The electrospinning production was optimized including polymer concentration and <br /> <br /> flow rate. The electrospinning process parameters selected were at 18 kV, the flow rate of 1 <br /> &#956;L/minutes, and the nozzle to collector distance of 10 cm. The uniform nanofibers were <br /> <br /> produced using solution concentration of 20% w/v and 40% of PVA-chitosan and gelatinchitosan, <br /> <br /> respectively. The maximum propolis incorporation in those nanofibers were 40% <br /> <br /> and 15%. PVA-Chitosan formula containing 30% propolis produced the best uniform <br /> <br /> nanofibers with average fiber diameter of 80.75±17.58 nm, porosity 47.19±17.31%, water <br /> <br /> absorption capacity of 442.5±10.61%, and antioxidant activity of IC50 is 81.04±3.03 ppm <br /> <br /> analyzed using DPPH. The nanofibers formula is promising for wound healing acceleration of <br /> <br /> severe skin wound.