#TITLE_ALTERNATIVE#

• Main Author: PRATIWI (NIM 10704045), ERLIN
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/21974
• Institution: Institut Teknologi Bandung

Saponin from Panax notogingseng had been studied possess hepatoprotective activity. Oyong (Luffa acutangula (L.) Roxb) had been investigated contain saponin compounds such as acutoside A-G. Purposes of this research are to develop the method of fractionation of saponin and to study of hepatoprotective activity of saponin fraction from oyong seeds (Luffa acutangula (L.) Roxb) in liver fibrositic rat induced with CCl4. The seeds of Luffa acutangula (L.) Roxb were extracted with ethanol 96% and fractionated with n-hexane and n-butanol. Saponin was identified with fish index and hemolytic index. Male Wistar rats were divided into six groups consist of normal group (given olive oil i.p. twice a week), control group (given CMC-Na 0,1% p.o. and CCl4 40% in olive oil i.p. twice a week), comparator group (given silymarin 0,625 g/kg bw p.o. and CCl4 40% in olive oil i.p. twice a week), and three test groups (given saponin fraction of Luffa acutangula (L.) Roxb at doses of 10 mg/kg bw p.o. twice a week, 20 mg/kg bw p.o. twice a week and 20 mg/kg bw p.o. once daily, respectively and CCl4 40% in olive oil i.p. twice a week) for eight weeks. The hepatoprotective activity were evaluated by measuring the activities of SGPT, SGOT, ALP, LDH and total bilirubin level. The fish index showed total saponin content of nbutanol fraction is bigger (12.500) than water fraction (1.250), n-hexane fraction (400) and ethanol extract (6.250). While hemolytic index also showed total saponin content of nbutanol fraction is bigger (2,00) than water fraction (1,33), n-hexane fraction (1,11) and ethanol extract (1,18). Administration of saponin fraction at dose of 10 mg/kg bw twice a week and 20 mg/kg bw twice a week and 20 mg/kg bw everyday can decrease the SGPT activity significantly compared to control group at 6th, 7th weeks, while did not show any significant differences at 8th weeks. In addition to administration of saponin fraction of treated groups also decrease the activities of SGOT, ALP, LDH and total bilirubin level but are not significant. Total saponin content of n-butanol fraction is the biggest of all fractions as well as extract. Administration of saponin fraction at doses of 10 mg/kg bw twice a week, 20 mg/kg bw twice a week, and 20 mg/kg bw once daily showed hepatoprotective activity in male Wistar rats induced with CCl4.