LACCASE PRODUCTION BY Marasmius sp. FROM OIL PALM EMPTY FRUIT BUNCH (OPEFB) USING TRICKLE-BED BIOREACTOR
Laccase is an enzyme that can be used in a variety of biotechnological processes, <br /> <br /> such as the bioremediation of phenol pollutants, dye decolorization on textile <br /> <br /> waste, and pulp bleaching. This is because laccase has a wide substrate specificity, &l...
Saved in:
Main Author: | |
---|---|
Format: | Theses |
Language: | Indonesia |
Online Access: | https://digilib.itb.ac.id/gdl/view/22054 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Institut Teknologi Bandung |
Language: | Indonesia |
Summary: | Laccase is an enzyme that can be used in a variety of biotechnological processes, <br />
<br />
such as the bioremediation of phenol pollutants, dye decolorization on textile <br />
<br />
waste, and pulp bleaching. This is because laccase has a wide substrate specificity, <br />
<br />
eco-friendly, and uses oxygen as the final electron acceptor. Laccase enzyme can <br />
<br />
be produced by white-rot fungi, i.e. Marasmius sp. by degrading lignin <br />
<br />
component of lignocellulose in agricultural waste, such as oil palm empty fruit <br />
<br />
bunches (OPEFB). Utilization of OPEFB can reduce enzyme production costs and <br />
<br />
answering high enzyme demand. Production of laccase enzyme by Marasmius sp. <br />
<br />
from OPEFB can be done using trickle-bed bioreactor with considering the <br />
<br />
aeration rate that may affect the growth and metabolic activity of Marasmius sp. <br />
<br />
The aim of study is to determine the optimum aeration rate (1,0; 1,5; and 2,0 vvm) <br />
<br />
in producing the highest activity of laccase using trickle-bed bioreactor. The <br />
<br />
enzyme production was performed using OPEFB with size of 1 cm and a <br />
<br />
temperature of 32ºC for 14 days. During enzyme production, the Kirk medium <br />
<br />
(initial pH 4,5) was trickled continuously into the bioreactor at a rate of 10 <br />
<br />
mL/min and 1,5 liters of the medium was added on day 1 to 4, day 7, and day 10. <br />
<br />
The effluent is taken every 12 hours for measurement of enzyme activity: laccase, <br />
<br />
lignin peroxidase (LiP), manganese peroxidase (MnP), and cellulose using the <br />
<br />
spectrophotometric method. In addition; pH, lignin content, and reducing sugars <br />
<br />
were also measured. The results showed that the highest to the lowest laccase <br />
<br />
activity was produced respectively by the aeration rate of 1.5 vvm (36.66 U/mL at <br />
<br />
the 84th hour); 1.0 vvm (23.80 U/mL at the 180th hour); and 2.0 vvm (19.36 U/mL <br />
<br />
at the 84th hour). Other than laccase; the activity of cellulase, LiP, and MnP was <br />
<br />
detected; but their activity was lower than laccase. Based on these results, it can <br />
<br />
be concluded that the aeration rate can affect the laccase activity and the aeration <br />
<br />
rate of 1.5 vvm is the optimum rate for laccase production. |
---|