THE EFFECT OF HARVEST AGE ON THE CONTENT OF ESSENTIAL OIL FROM 'NILAM' (Pogostemon cablin Benth.) SHOOT CULTURE ON THIN LAYER CULTURE SYSTEM (TLC)

Pogostemon cablin Benth. or better known as patchouli is one of the leading varieties in Indonesia with the highest content of patchouli alcohol compounds compared with other varieties. Patchouli alcohol compounds contained in patchouli oil and is a factor that determines the quality and price of pa...

Full description

Saved in:
Bibliographic Details
Main Author: AMADEA (NIM : 11213031), GRACE
Format: Final Project
Language:Indonesia
Online Access:https://digilib.itb.ac.id/gdl/view/22300
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Institut Teknologi Bandung
Language: Indonesia
id id-itb.:22300
spelling id-itb.:223002017-09-27T11:26:41ZTHE EFFECT OF HARVEST AGE ON THE CONTENT OF ESSENTIAL OIL FROM 'NILAM' (Pogostemon cablin Benth.) SHOOT CULTURE ON THIN LAYER CULTURE SYSTEM (TLC) AMADEA (NIM : 11213031), GRACE Indonesia Final Project INSTITUT TEKNOLOGI BANDUNG https://digilib.itb.ac.id/gdl/view/22300 Pogostemon cablin Benth. or better known as patchouli is one of the leading varieties in Indonesia with the highest content of patchouli alcohol compounds compared with other varieties. Patchouli alcohol compounds contained in patchouli oil and is a factor that determines the quality and price of patchouli oil. The existing cultivation method in Indonesia has not been efficient to produce patchouli oil with a minimum of 30% patchouli alcohol content, as a requirement for the patchouli oil traded in the world. One alternative method that can be used for patchouli propagation in controlled environment is the in vitro shoot culture method in thin layer culture (TLC) system. The study was conducted to determine the effect of harvest time on the essential oil content of plant shoots culture and to evaluate the bioconversion of medium substrates into shoot biomass. In this study, patchouli shoots were grown on Murashige and Skoog (MS) media without the addition of growth regulators. This shoot culture was cultivated for 28 days with harvest time variation every 7 days. The growth rate of biomass was 0.054 mg DW/day with doubling time of 12.78 days. The value of the sucrose content and the conductivity of the medium were correlated with the rate of increase in biomass. The yield of patchouli oil obtained ranged from 2.34 – 3.23%. The oil content found on Gas Chromatography-Mass Spectrometry (GC-MS) analysis was farnesane (0,18% - 0,28%), eicosane (1,17% - 4,24%), squalene (0,35% - 0,46%), and most groups of alkanes and fatty acids, however no patchouli alcohol compound was found in the tested oil sample. text
institution Institut Teknologi Bandung
building Institut Teknologi Bandung Library
continent Asia
country Indonesia
Indonesia
content_provider Institut Teknologi Bandung
collection Digital ITB
language Indonesia
description Pogostemon cablin Benth. or better known as patchouli is one of the leading varieties in Indonesia with the highest content of patchouli alcohol compounds compared with other varieties. Patchouli alcohol compounds contained in patchouli oil and is a factor that determines the quality and price of patchouli oil. The existing cultivation method in Indonesia has not been efficient to produce patchouli oil with a minimum of 30% patchouli alcohol content, as a requirement for the patchouli oil traded in the world. One alternative method that can be used for patchouli propagation in controlled environment is the in vitro shoot culture method in thin layer culture (TLC) system. The study was conducted to determine the effect of harvest time on the essential oil content of plant shoots culture and to evaluate the bioconversion of medium substrates into shoot biomass. In this study, patchouli shoots were grown on Murashige and Skoog (MS) media without the addition of growth regulators. This shoot culture was cultivated for 28 days with harvest time variation every 7 days. The growth rate of biomass was 0.054 mg DW/day with doubling time of 12.78 days. The value of the sucrose content and the conductivity of the medium were correlated with the rate of increase in biomass. The yield of patchouli oil obtained ranged from 2.34 – 3.23%. The oil content found on Gas Chromatography-Mass Spectrometry (GC-MS) analysis was farnesane (0,18% - 0,28%), eicosane (1,17% - 4,24%), squalene (0,35% - 0,46%), and most groups of alkanes and fatty acids, however no patchouli alcohol compound was found in the tested oil sample.
format Final Project
author AMADEA (NIM : 11213031), GRACE
spellingShingle AMADEA (NIM : 11213031), GRACE
THE EFFECT OF HARVEST AGE ON THE CONTENT OF ESSENTIAL OIL FROM 'NILAM' (Pogostemon cablin Benth.) SHOOT CULTURE ON THIN LAYER CULTURE SYSTEM (TLC)
author_facet AMADEA (NIM : 11213031), GRACE
author_sort AMADEA (NIM : 11213031), GRACE
title THE EFFECT OF HARVEST AGE ON THE CONTENT OF ESSENTIAL OIL FROM 'NILAM' (Pogostemon cablin Benth.) SHOOT CULTURE ON THIN LAYER CULTURE SYSTEM (TLC)
title_short THE EFFECT OF HARVEST AGE ON THE CONTENT OF ESSENTIAL OIL FROM 'NILAM' (Pogostemon cablin Benth.) SHOOT CULTURE ON THIN LAYER CULTURE SYSTEM (TLC)
title_full THE EFFECT OF HARVEST AGE ON THE CONTENT OF ESSENTIAL OIL FROM 'NILAM' (Pogostemon cablin Benth.) SHOOT CULTURE ON THIN LAYER CULTURE SYSTEM (TLC)
title_fullStr THE EFFECT OF HARVEST AGE ON THE CONTENT OF ESSENTIAL OIL FROM 'NILAM' (Pogostemon cablin Benth.) SHOOT CULTURE ON THIN LAYER CULTURE SYSTEM (TLC)
title_full_unstemmed THE EFFECT OF HARVEST AGE ON THE CONTENT OF ESSENTIAL OIL FROM 'NILAM' (Pogostemon cablin Benth.) SHOOT CULTURE ON THIN LAYER CULTURE SYSTEM (TLC)
title_sort effect of harvest age on the content of essential oil from 'nilam' (pogostemon cablin benth.) shoot culture on thin layer culture system (tlc)
url https://digilib.itb.ac.id/gdl/view/22300
_version_ 1821120728818253824