#TITLE_ALTERNATIVE#
Pathogenic bacteria was a kind of microorganism which could caused infection disease. Infection <br /> <br /> disease would be treated difficullty if bacteria had undergone antibiotic resistance. The search for new <br /> <br /> antibiotics continues to be done to overcome th...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/25112 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Pathogenic bacteria was a kind of microorganism which could caused infection disease. Infection <br />
<br />
disease would be treated difficullty if bacteria had undergone antibiotic resistance. The search for new <br />
<br />
antibiotics continues to be done to overcome the problem. Indonesia as the largest maritime country <br />
<br />
had a potency to discover a new antibiotic from marine sources. The aim of research was to isolate <br />
<br />
and determine antibacterial activity of secondary metabolite of Trichoderma longibrachiatum derived <br />
<br />
from sea sponge. Trichoderma longibrachiatum was fermented with and without shaking. The result <br />
<br />
of fermentation was filtered to separate mycelium and filtrate. Mycelium was extracted with <br />
<br />
maceration methode while filtrate was extracted with liquid-liquid extraction method. The mycelium <br />
<br />
and filtrate extract were monitored with TLC and agar diffusion antibacterial assay. Mycelium extract <br />
<br />
had better antibacterial activity with inhibition zone 15 mm against Staphylococcus aureus and <br />
<br />
inhibition zone 11.4 mm against Escherichia coli. Mycelium extracts were fractionated with classical <br />
<br />
column chromatography. Fraction 2 and 3 had antibacterial activity against Staphylococcus aureus and <br />
<br />
Escherichia coli. Combined fraction of 2 dan 3 was done further fractination. Subfractions were test for <br />
<br />
antibacterial activity with agar difusion against Escherichia coli and Staphylococcus aureus. Subfraction <br />
<br />
1, 3, 4, and 6 had antibacterial activity against Staphylococcus aureus with inhibition zone 7.3, 7.45, <br />
<br />
7.1, and 9.6 mm. Because of that subfraction of T. longibrachiatum’s secondary metabolite had a <br />
<br />
potency to be an antibiotic. <br />
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