#TITLE_ALTERNATIVE#

• Main Author: S. MUHARAM NIM : 10714060 , AGNIA
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/25112
• Institution: Institut Teknologi Bandung

Pathogenic bacteria was a kind of microorganism which could caused infection disease. Infection <br /> <br /> disease would be treated difficullty if bacteria had undergone antibiotic resistance. The search for new <br /> <br /> antibiotics continues to be done to overcome the problem. Indonesia as the largest maritime country <br /> <br /> had a potency to discover a new antibiotic from marine sources. The aim of research was to isolate <br /> <br /> and determine antibacterial activity of secondary metabolite of Trichoderma longibrachiatum derived <br /> <br /> from sea sponge. Trichoderma longibrachiatum was fermented with and without shaking. The result <br /> <br /> of fermentation was filtered to separate mycelium and filtrate. Mycelium was extracted with <br /> <br /> maceration methode while filtrate was extracted with liquid-liquid extraction method. The mycelium <br /> <br /> and filtrate extract were monitored with TLC and agar diffusion antibacterial assay. Mycelium extract <br /> <br /> had better antibacterial activity with inhibition zone 15 mm against Staphylococcus aureus and <br /> <br /> inhibition zone 11.4 mm against Escherichia coli. Mycelium extracts were fractionated with classical <br /> <br /> column chromatography. Fraction 2 and 3 had antibacterial activity against Staphylococcus aureus and <br /> <br /> Escherichia coli. Combined fraction of 2 dan 3 was done further fractination. Subfractions were test for <br /> <br /> antibacterial activity with agar difusion against Escherichia coli and Staphylococcus aureus. Subfraction <br /> <br /> 1, 3, 4, and 6 had antibacterial activity against Staphylococcus aureus with inhibition zone 7.3, 7.45, <br /> <br /> 7.1, and 9.6 mm. Because of that subfraction of T. longibrachiatum’s secondary metabolite had a <br /> <br /> potency to be an antibiotic. <br />