ENHANCEMENT OF LUTEIN CONTENT IN Chlorella pyrenoidosa CULTURE UNDER SALINITY STRESS CONDITION

Chlorella pyrenoidosa is a promising alternative resource of lutein and zeaxanthin production. Low levels of lutein and zeaxanthin in culture cause expensive production processes. The aim of this study is to increase the productivity of lutein and zeaxanthin in culture through induction of salinity...

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Main Author: Dwi Guno Hutomo (Nim : 11214029), Agung
Format: Final Project
Language:Indonesia
Online Access:https://digilib.itb.ac.id/gdl/view/25122
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Institution: Institut Teknologi Bandung
Language: Indonesia
id id-itb.:25122
spelling id-itb.:251222018-09-26T15:25:15ZENHANCEMENT OF LUTEIN CONTENT IN Chlorella pyrenoidosa CULTURE UNDER SALINITY STRESS CONDITION Dwi Guno Hutomo (Nim : 11214029), Agung Indonesia Final Project INSTITUT TEKNOLOGI BANDUNG https://digilib.itb.ac.id/gdl/view/25122 Chlorella pyrenoidosa is a promising alternative resource of lutein and zeaxanthin production. Low levels of lutein and zeaxanthin in culture cause expensive production processes. The aim of this study is to increase the productivity of lutein and zeaxanthin in culture through induction of salinity stress. In this research, a kinetic model for lutein and zeaxanthin production was developed based on batch cultivation in 0.9 L bioreactor. Two stage cultivation process was developed to increase lutein and zeaxanthin productivity. Chlorella pyrenoidosa culture was given salinity stress treatment in the form of adding NaCl concentration of 0.05, 0.1, and 0.2 g L-1 and salinity stress induction on day 0, 7 and 11 of cultivation. Cultivation for 12 days showed that giving salinity stress can increase carotenoid levels in cells but have a negative impact inhibiting growth microalga cells. Under salinity stress condition, Chlorella pyrenoidosa produced up to 9 and 6,7 mg g-1 lutein and zeaxanthin. It also showed that salinity stress conditions are more favorable for carotenoid production with 92.3 and 94.7% increase of lutein and zeaxanthin productivity. text
institution Institut Teknologi Bandung
building Institut Teknologi Bandung Library
continent Asia
country Indonesia
Indonesia
content_provider Institut Teknologi Bandung
collection Digital ITB
language Indonesia
description Chlorella pyrenoidosa is a promising alternative resource of lutein and zeaxanthin production. Low levels of lutein and zeaxanthin in culture cause expensive production processes. The aim of this study is to increase the productivity of lutein and zeaxanthin in culture through induction of salinity stress. In this research, a kinetic model for lutein and zeaxanthin production was developed based on batch cultivation in 0.9 L bioreactor. Two stage cultivation process was developed to increase lutein and zeaxanthin productivity. Chlorella pyrenoidosa culture was given salinity stress treatment in the form of adding NaCl concentration of 0.05, 0.1, and 0.2 g L-1 and salinity stress induction on day 0, 7 and 11 of cultivation. Cultivation for 12 days showed that giving salinity stress can increase carotenoid levels in cells but have a negative impact inhibiting growth microalga cells. Under salinity stress condition, Chlorella pyrenoidosa produced up to 9 and 6,7 mg g-1 lutein and zeaxanthin. It also showed that salinity stress conditions are more favorable for carotenoid production with 92.3 and 94.7% increase of lutein and zeaxanthin productivity.
format Final Project
author Dwi Guno Hutomo (Nim : 11214029), Agung
spellingShingle Dwi Guno Hutomo (Nim : 11214029), Agung
ENHANCEMENT OF LUTEIN CONTENT IN Chlorella pyrenoidosa CULTURE UNDER SALINITY STRESS CONDITION
author_facet Dwi Guno Hutomo (Nim : 11214029), Agung
author_sort Dwi Guno Hutomo (Nim : 11214029), Agung
title ENHANCEMENT OF LUTEIN CONTENT IN Chlorella pyrenoidosa CULTURE UNDER SALINITY STRESS CONDITION
title_short ENHANCEMENT OF LUTEIN CONTENT IN Chlorella pyrenoidosa CULTURE UNDER SALINITY STRESS CONDITION
title_full ENHANCEMENT OF LUTEIN CONTENT IN Chlorella pyrenoidosa CULTURE UNDER SALINITY STRESS CONDITION
title_fullStr ENHANCEMENT OF LUTEIN CONTENT IN Chlorella pyrenoidosa CULTURE UNDER SALINITY STRESS CONDITION
title_full_unstemmed ENHANCEMENT OF LUTEIN CONTENT IN Chlorella pyrenoidosa CULTURE UNDER SALINITY STRESS CONDITION
title_sort enhancement of lutein content in chlorella pyrenoidosa culture under salinity stress condition
url https://digilib.itb.ac.id/gdl/view/25122
_version_ 1822020596374962176