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Colon cancer is the third type of cancer with the most number of patient in the world, <br /> <br /> which caused 694,000 death. From some data research, Mangosteen rind contain <br /> <br /> some substances with pharmacological activities such as antihistamines, <br />...
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| Format: | Theses |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/25597 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Colon cancer is the third type of cancer with the most number of patient in the world, <br />
<br />
which caused 694,000 death. From some data research, Mangosteen rind contain <br />
<br />
some substances with pharmacological activities such as antihistamines, <br />
<br />
anti-inflammatory, antioxidants, cardiovascular medicines, antimicroorganism dan <br />
<br />
also as anti cancer. Main substance reported having pharmacological activities is <br />
<br />
xanton group. Xanton has an cytotoxicity and antiproliferation effect which could <br />
<br />
inhibit and kill cancer cell. The most Xanton compound contained in Mangosteen are <br />
α-mangostin and γ-mangostin. α-mangostin is a compound which has high efficacy in <br />
<br />
suppressing carcinogen compound formation in the colon. While γ-mangostin has a <br />
<br />
lot of benefit in protection against the disease. In vitro test pro-apoptosis and <br />
<br />
anti-proliferative xanton in Mangosteen to COLO205 (human colorectal <br />
<br />
adenocarcinoma) cell shown apoptosys induction effect, activation caspase-3 and -8, <br />
<br />
and mitocondria cytokrom c release. Then in vivo test of antitumor xanton to <br />
<br />
COLO205 (human colorectal adenocarcinoma) shown growth inhibition effect on 3 <br />
<br />
mg extract / tumor, apoptosys cell, nuclear fragmentation and cromatin condensation, <br />
<br />
and caspases-3 and -8 activation. This research will be conducted biodistribution test <br />
<br />
of α, γmangosteen which has developed before to observe the distribution profile <br />
<br />
inside the body especially blood and other organs where examined animals used are <br />
<br />
mice (Mus musculus) stock Balb/c. Colorectal cancer mice speciment obtained <br />
<br />
through single dose 10 mg/kg bb (i.p) of azoxymethane induction and dextran sodium <br />
<br />
sulfate 2.5% in the drinking water. The method used is substance marker of α, γ <br />
<br />
mangosteen with radionuclide131 I as a tracer to find out the biodistribution inside the <br />
<br />
body. Highest purity of compound marker131 I-α, γMangosteen syntesis is on formula <br />
<br />
3 (F3) with level concentration α, γMangosteen 500 mg is 91,27% and pH 7. <br />
<br />
Biodistribution observation has been conducted to each group after 1, 4, and 24 <br />
<br />
hours after oral administration compound marker <br />
<br />
131 <br />
<br />
I-α, γMangosteen. Compound <br />
<br />
marker <br />
<br />
131 <br />
<br />
I-α, γMangosteen relative accumulation increasing in cancer location <br />
<br />
tissue counted by comparing % radioactivity of cancer tissue to % radioactivity of the <br />
<br />
same tissue of healthy animal. There are difference in relative accumulation value of <br />
α, γmangosteen marker compound on cancer location compared to normal tissue, <br />
<br />
where T/NT ratio value of normal speciment group is higher compared to cancer <br />
<br />
speciment group. It shows accumulations occurs of compound marker <br />
<br />
131 <br />
<br />
I-α, γ <br />
<br />
Mangosteen in the animal group with cancer speciment, but not significant. Half life <br />
<br />
of compound marker131 I-α, γMangosteen in the animal group with cancer speciment <br />
<br />
is longer than normal speciment group. Absorption half life is 2,52 hour, <br />
<br />
biodistribution half life is 19,97 hour, and elimination half life is 21,98 hour. <br />
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