#TITLE_ALTERNATIVE#

• Main Author: RESTIASARI NIM: 20715034, ANGGI
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/25597
• Institution: Institut Teknologi Bandung

Colon cancer is the third type of cancer with the most number of patient in the world, <br /> <br /> which caused 694,000 death. From some data research, Mangosteen rind contain <br /> <br /> some substances with pharmacological activities such as antihistamines, <br /> <br /> anti-inflammatory, antioxidants, cardiovascular medicines, antimicroorganism dan <br /> <br /> also as anti cancer. Main substance reported having pharmacological activities is <br /> <br /> xanton group. Xanton has an cytotoxicity and antiproliferation effect which could <br /> <br /> inhibit and kill cancer cell. The most Xanton compound contained in Mangosteen are <br /> &#945;-mangostin and &#947;-mangostin. &#945;-mangostin is a compound which has high efficacy in <br /> <br /> suppressing carcinogen compound formation in the colon. While &#947;-mangostin has a <br /> <br /> lot of benefit in protection against the disease. In vitro test pro-apoptosis and <br /> <br /> anti-proliferative xanton in Mangosteen to COLO205 (human colorectal <br /> <br /> adenocarcinoma) cell shown apoptosys induction effect, activation caspase-3 and -8, <br /> <br /> and mitocondria cytokrom c release. Then in vivo test of antitumor xanton to <br /> <br /> COLO205 (human colorectal adenocarcinoma) shown growth inhibition effect on 3 <br /> <br /> mg extract / tumor, apoptosys cell, nuclear fragmentation and cromatin condensation, <br /> <br /> and caspases-3 and -8 activation. This research will be conducted biodistribution test <br /> <br /> of &#945;, &#947;mangosteen which has developed before to observe the distribution profile <br /> <br /> inside the body especially blood and other organs where examined animals used are <br /> <br /> mice (Mus musculus) stock Balb/c. Colorectal cancer mice speciment obtained <br /> <br /> through single dose 10 mg/kg bb (i.p) of azoxymethane induction and dextran sodium <br /> <br /> sulfate 2.5% in the drinking water. The method used is substance marker of &#945;, &#947; <br /> <br /> mangosteen with radionuclide131 I as a tracer to find out the biodistribution inside the <br /> <br /> body. Highest purity of compound marker131 I-&#945;, &#947;Mangosteen syntesis is on formula <br /> <br /> 3 (F3) with level concentration &#945;, &#947;Mangosteen 500 mg is 91,27% and pH 7. <br /> <br /> Biodistribution observation has been conducted to each group after 1, 4, and 24 <br /> <br /> hours after oral administration compound marker <br /> <br /> 131 <br /> <br /> I-&#945;, &#947;Mangosteen. Compound <br /> <br /> marker <br /> <br /> 131 <br /> <br /> I-&#945;, &#947;Mangosteen relative accumulation increasing in cancer location <br /> <br /> tissue counted by comparing % radioactivity of cancer tissue to % radioactivity of the <br /> <br /> same tissue of healthy animal. There are difference in relative accumulation value of <br /> &#945;, &#947;mangosteen marker compound on cancer location compared to normal tissue, <br /> <br /> where T/NT ratio value of normal speciment group is higher compared to cancer <br /> <br /> speciment group. It shows accumulations occurs of compound marker <br /> <br /> 131 <br /> <br /> I-&#945;, &#947; <br /> <br /> Mangosteen in the animal group with cancer speciment, but not significant. Half life <br /> <br /> of compound marker131 I-&#945;, &#947;Mangosteen in the animal group with cancer speciment <br /> <br /> is longer than normal speciment group. Absorption half life is 2,52 hour, <br /> <br /> biodistribution half life is 19,97 hour, and elimination half life is 21,98 hour. <br />