#TITLE_ALTERNATIVE#

• Main Author: WAHYU ROHMADHANI NIM : 10714061, DWI
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/26803
• Institution: Institut Teknologi Bandung

Free radicals are molecules that have a group of atoms with unpaired electrons, so free radicals are <br /> <br /> reactive and unstable. Antioxidants are compounds that work by donating an electron to an oxidant <br /> <br /> compound so the activity of the oxidant compound can be inhibited. Antioxidant compounds such <br /> <br /> as phenolic and flavonoid compounds can reduce and protect cells from damaging. Many plants <br /> <br /> contain phenolic and flavonoid compounds included pumpkin. The objectives of this research were <br /> <br /> to study antioxidant activity of flesh, peel, and seed of pumpkin by determining IC50 of DPPH (2,2- <br /> <br /> diphenyl-1-picrylhydrazyl) and EC50 of CUPRAC (Cupric Reducing Antioxidant Capacity); total <br /> <br /> phenolic and flavonoid content; analyze the correlation between total phenolic and flavonoid with <br /> <br /> their IC50 DPPH and EC50 FRAP, and analyze the correlation beetwen DPPH and FRAP in sample <br /> <br /> extracts. Extraction was performed by reflux using gradient polarity solvents. Determination of IC50 <br /> <br /> DPPH, CUPRAC EC50 capacity, total phenolic and flavonoid content of each extract were performed <br /> <br /> by spectrophotometry uv-visible and correlation of total phenolic and flavonoid content with their <br /> <br /> IC50 of DPPH and EC50 &#272;» &#1048614;&#1048666;!&#1048663; ®&#292;ð&#330;ð&#292;ð±&#282;&#331;±&#278;±  &#265; &#258;&#337;&#1049036;±¸ ­&#337;&#1048663;± &#278;&#282;&#272;&#265;&#1049435;&#282;&#264;±&#292;í&#272;¸. N-hexane peel extract <br /> <br /> of pumpkin had the lowest IC50 DPPH (5.55 ± 0.10 µg/mL) and ethyl acetate seeds extract of <br /> <br /> pumpkin EC50 CUPRAC (104.17 ± 4.37 µg/mL ). The highest total phenolic content was given by the <br /> <br /> ethyl acetate peel extract of pumpkin (4.09 ± 0.10 g GAE/100 g), while the n-hexane flesh extract <br /> <br /> of pumpkin showed the highest total flavonoid content (11.22 ± 0.28 g QE/100 g). Total phenolic <br /> <br /> content in seed extracts of pumpkin had negative and significant correlation with their EC50 <br /> <br /> CUPRAC, while total flavonoid content in flesh, peel and seeds extracts of pumpkin had negative <br /> <br /> and significant correlation with their EC50 CUPRAC. IC50 DPPH of flesh and peel extracts of pumpkin <br /> <br /> had significantly positive correlation with their EC50 CUPRAC. All organs extract of pumpkin were <br /> <br /> categorized as very strong antioxidant activity by DPPH method, hence it was potential to be <br /> <br /> developed as source of natural antioxidant. Phenolic compounds were the major contributors in <br /> <br /> antioxidant activity of seeds extracts of pumpkin by CUPRAC method. Flavonoid compounds were <br /> <br /> the major contributors in antioxidant activity of flesh, peel and seeds extracts of pumpkin by <br /> <br /> CUPRAC method. DPPH and CUPRAC methods gave linear result in antioxidant activity of flesh and <br /> <br /> peel extracts of pumpkin. <br />