#TITLE_ALTERNATIVE#

• Main Author: MUSTIKA NINGRUM NIM 10714079, ELEN
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/26896
• Institution: Institut Teknologi Bandung

Free radical is compound which has unpaired electron on their outer orbital, so it became reactive. <br /> <br /> An antioxidant is a compound that donates electrons so it can inactivate the development of <br /> <br /> oxidation reactions by preventing the formation of free radical. A lot of plants having antioxidant <br /> <br /> activities, one of them is kepundung (Baccaurea racemosa Müll.Arg.). Leaves of kepundung use to <br /> <br /> cure constipation and facilitate woman through the menstruation period traditionally. The <br /> <br /> objectives of this research were to identify the chemical compound from the leaves of Baccaurea <br /> <br /> racemosa Müll.Arg., study the antioxidant activity of kepundung leaves through the determination <br /> <br /> of IC50 scavenging free radicals activity DPPH, to isolate the antioxidant compound, and to <br /> <br /> determine the active compound; fenol and flavonoid total content. Crude drugs of kepundung <br /> <br /> leaves were extracted by the reflux method using ethanol as the solvent. The value of IC50 of DPPH <br /> <br /> scavenging activity, total phenolic content, and total flavonoid content were 1,86 ± 0,18 µg/mL; <br /> <br /> 2,36 ± 0,15 g GAE/100 g; 1,93 ± 0,02 g QE/100 g. Extracts were continued to fractionation by liquidliquid extraction using n-hexane and ethyl acetate as solvents. Ethyl acetate fraction was <br /> <br /> subfractionated using radial chromatography. Subfraction 12 until 50 were purified using TLC <br /> <br /> preparative. The purity was observed using three different mobile phases of TLC. The isolate was <br /> <br /> characterized using specific spot reagent, ammonia vapour, and spectrophotodensitometry. The <br /> <br /> isolate was predicted to be an isoflavone lacking a free 5-hydroxyl group with maximum <br /> <br /> absorbances at 263 nm and 328 nm. <br /> <br />