ISOLATION AND IDENTIFICATION OF FATTY ACID AND THE DERIVATIVES FROM BITTER MELON (MOMORDICA CHARANTIA) SEED

• Main Author: SAPUTRA (NIM:10514076), JAYA
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/28236
• Institution: Institut Teknologi Bandung

Indonesia is one of many tropical countries endowed with a bunch variety of flora. Bitter melon <br /> <br /> (Momordica charantia) is one plant out of tons which is thriving in Indonesia. It is commonly <br /> <br /> utilized as food and traditional medicine. One group of compound that is biologically active in <br /> <br /> bitter melon is its unsaturated fatty acid. Unsaturated fatty acid in bitter melon was reported to be <br /> <br /> able to suppress cancer growth and stimulate lipid metabolism in human body. To get a full <br /> <br /> benefit from bitter melon, phytochemical research is needed. In this project, fatty acid and its <br /> <br /> derivatives were isolated from 0.520 kg of dry bitter melon seeds. The seeds were macerated <br /> <br /> with methanol (3x24 hours). Fractionation of methanol extract was done by the means of liquid <br /> <br /> vacuum chromatography, radial chromatography, and gravity column chromatography. Each <br /> <br /> isolated compound was characterized with 1H-NMR and 13C-NMR. In this project, there are <br /> <br /> three compounds that were managed to be isolated and characterized. They are &#945;-eleostearic <br /> <br /> acid, glyceryl-1,3-di-&#945;-eleostearate-2-behenate and glyceryl-1,2-&#945;-palmtitate