#TITLE_ALTERNATIVE#

• Main Author: NAADIYATUL HUDA NIM : 11614038, KHAULAH
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/28479
• Institution: Institut Teknologi Bandung

Thrombosis is the formation of blood clots in vessels that involve the process of hemostasis. <br /> <br /> Almost all cardiovascular diseases have a correlation with thrombosis. Traditionally binahong, <br /> <br /> kemuning, and sambiloto have blood cleansing functions. This study was conducted to determine <br /> <br /> the antithrombotic activity of binahong, kemuning, and sambiloto extracts in vivo. Extracts were <br /> <br /> obtained by maceration method using 96% ethanol. Antithrombotic activity was carried out by <br /> <br /> measuring bleeding time, coagulation time, total bleeding, and bleeding pattern using male Swiss- <br /> <br /> Webster mice. In the bleeding time and coagulation time test, the mice were divided into nine <br /> <br /> groups, namely the normal group, the aspirin group, the warfarin group, and the extract group <br /> <br /> with doses of 140 mg/kg and 280 mg/kg, respectively. Time of bleeding, coagulation time, total <br /> <br /> bleeding, and bleeding pattern was measured 30 minutes after administration of the test <br /> <br /> substance on day 3. From the results of the bleeding time and coagulation time, kemuning extract <br /> <br /> 140 mg/kg and 280 mg/kg significantly extended both parameters and sambiloto extract 280 <br /> <br /> mg/kg extended the bleeding time. When bleeding and normal group coagulation time, kemuning <br /> <br /> extract 140 mg/kg and 280 mg/kg respectively were 55.33 ± 2.52 and 56.83 ± 4.65 seconds, 86 ± <br /> <br /> 12.16 and 77.33 ± 19, 53 seconds, 167 ± 40.04 and 89.67 ± 5.62 seconds, and bleeding time of <br /> <br /> sambiloto extract 280 mg/kg is 86.67 ± 19.86 seconds. From the results of the total bleeding test, <br /> <br /> binahong extract 280 mg/kg and kemuning extract 280 mg/kg increased the total hemoglobin <br /> <br /> level with a value of 14.21 ± 3.53 g/dl and 27.92 ± 4.99 g/dl compared to the normal group with a <br /> <br /> total hemoglobin level of 0.91 ± 0.41 g/dl. The bleeding pattern shows rebleeding of binahong <br /> <br /> extract 280 mg/kg group in the 15th minute and kemuning extract 280 mg/kg in the 25th minute. <br /> <br /> Kemuning extract 280 mg/kg gave the best antithrombotic activity in all parameters tested. <br /> <br /> Binahong extract 280 mg/kg tends to be antithrombotic in total bleeding test. Sambiloto extract <br /> <br /> 280 mg/kg antithrombotic activity only observed in bleeding time. <br />