#TITLE_ALTERNATIVE#

• Main Author: Diana , Michelle
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/28908
• Institution: Institut Teknologi Bandung

Plant tissue in Purple Sweet Potato (Ipomoea batatas L.) is known to produce phenolic compounds that have potential to be used as antioxidants. Researches on Purple Sweet Potato in vitro callus culture have been done to further analyze the phenolic content and antioxidants properties in Purple Sweet Potato. So far, those researches were still limited to the usage of tubers as the source of explant, while the other plant parts such as leaves and petioles are still not widely utilized. Therefore, this study was conducted with the aim: 1) to optimize the growth of Purple Sweet Potato callus induced from leaves (KD) and petioles (KP) on Murashige and Skoog (MS) culture medium by combination of BAP and 2,4-D; 2) to analyze the total phenolic content of Purple Sweet Potato callus cultures; and 3) to analyze the total antioxidant activity of the Purple Sweet Potato callus culture. The research was conducted through 3 stages, which are the callus induction phase on the variation of the medium, the subculture of callus on the optimization medium phase, and the analysis phase. Callus induction stage serves as a medium optimization stage to determine the best medium for the growth of Purple Sweet Potato callus for 4 weeks. The medium used in this step consisted of 15 combinations medium that were added with BAP at concentrations of 0, 10, and 15 ?M and 2,4-D at concentration of 0;0.5; 1; 2.5; and 5 ?M. The parameters that are used at this stage are the increasing amount of callus biomass, callus structure, and callus color. The callus subculture stage is performed for 4 weeks with the same observation parameters using three best medium combinations from previous stage. The total phenolic content of the callus was analyzed using the Folin-Ciocalteu method and expressed in the mg GAE/g dry weight, whereas the antioxidant activity of callus tissue was determined against the free radical compound of 1,1-diphenyl-2-picryl hydrazyl (DPPH) expressed in 1/IC50 value. Based on the research, it can be concluded that the leaf and petiole of Purple Sweet Potato can induce callus formation with the largest increase of KD biomass produced in callus maintained on medium with 10 ?M BAP and 5 ?M 2,4-D with increased biomass of 304,44 mg. Meanwhile, the largest biomass increased on KP was produced on medium with a combination of 10 ?M BAP without 2,4-D with biomass increase of 331,11 mg. The result showed that KD and KP contained the largest total phenolic compound in MS medium without added plant growth hormone at 2.62 ± 0.10 dan 1.62 ± 0.09 mg GAE/g dry weight respectively, and had compounds that could act as antioxidants with the largest 1/IC50 value on callus grown on 10 ?M BAP medium without 2,4-D at 1.78 ± 0.31 L/g dan 1.72 ± 0.26 L/g respectively.