INTERACTION COMPLEX CU (II) ˗SALISILALDEHYDE DINITROFENILHYDRAZON WITH PLASMID DNA PET˗32B
The bioactivity study of transition metal complex compounds with schiff base ligand is studied by many <br /> <br /> <br /> <br /> researchers, including its potential as antibacterial, antimicrobial, anticancer, and antioxidant. In this <br /> <br /> <...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/29733 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | The bioactivity study of transition metal complex compounds with schiff base ligand is studied by many <br />
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researchers, including its potential as antibacterial, antimicrobial, anticancer, and antioxidant. In this <br />
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reasearch studied the interaction between complex with Schiff base ligand, Cu(II)˗salisilaldehide <br />
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dinitrophenylhydrazone(SDPH) and DNA plasmid pET˗32b in vitro. The ligand SDPH has been <br />
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successfully synthesized by the condensation reaction between salicylaldehyde and <br />
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2,4˗dinitrophenylhydrazone, which showed a functional group C=N peak in the infrared spectrum. In the <br />
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1H NMR spectrum we observed proton signals at a shift value of 11,57 ppm (N˗H); 9,95 ppm (O˗H); 8,9 <br />
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ppm (C=N); and 7,0˗7,9 ppm (aromatic) corresponding to the structure of the SDPH. Cu(II)˗SDPH <br />
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complexes have been obtained by the reaction of Cu(C2H3O2)2.H2O and SDPH ligands by a ratio of 1: 1 <br />
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in the ethanol solvent. The coordination link between the Cu(II) ions and the SDPH ligand is indicated by <br />
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the peak shift of the ˗C=N functional group and the peak loss of the ˗OH group of the SDPH ligand. The <br />
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Cu(II) ˗SDPH complex has a maximum absorption at 381 nm and 519 nm in acetonitryl, 495 nm in <br />
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DMSO, 408 nm and 521 nm in BaSO4 solid and is a paramagnetic complex with a magnetic moment <br />
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value of 1,98 BM. The results of the study of the bioactivity of the cleaving of DNA by Cu(II)˗SDPH <br />
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with variations in concentration shows that the complex can cleave DNA, which is shown by the <br />
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percentage of form II and III which increase as concentration increases.The Cu(II)˗SDPH complex has <br />
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the potential as an anticancer.c |
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