#TITLE_ALTERNATIVE#

• Main Author: JUMMAH NIM: 20716007, NURUL
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/29839
• Institution: Institut Teknologi Bandung

Malaria caused by parasites is a major cause of child morbidity and mortality. <br /> <br /> Plasmodium falciparum is the most common cause of malaria, especially in <br /> <br /> eastern Indonesia. The problem of treatment of malaria with conventional <br /> <br /> medicine is drug resistance (multiple drug resistance) and not specific targets to <br /> <br /> intracellular parasites. The existence of resistance by conventional drugs in <br /> <br /> malaria parasites is caused by the use of antimalarial dosage forms that are less <br /> <br /> effective and the bioavailability of drugs that are low in high parasite amounts <br /> <br /> resulting in the need for high doses for therapeutic success and cause high <br /> <br /> toxicity. In this study, we purpose chitosan nanoparticles as vehicles to carry as- <br /> <br /> ODN targeting two genes, EBA-175 (Erythrocyte Binding Antigen-175 kD) and <br /> <br /> dhs (deoxyhypusine shynthase). Poloxamer was used in formulations for long <br /> <br /> circulating effect. Thus concentration of poloxamer used was 10% (w/v). <br /> <br /> Chitosan-poloxamer nanoparticles were made by ionic gelation method with <br /> <br /> sodium tripolyphosphate (STPP) as crosslinker. The results of the <br /> <br /> characterization of nanoparticle size obtained from this study was 93.3-172.5 nm <br /> <br /> (under 200 nm) with a polydispersity index 0.254-0.368 (under 0.5). The potential <br /> <br /> zeta measurement results 31.8–43.3 mV (above +30 mV) which shows the stability <br /> <br /> of the formulation made. This characterization was then confirmed by TEM <br /> <br /> measurements which showed spherical particle shape with a size below 200 nm. <br /> <br /> The highest inhibition of parasitemia was shown by chitosan-poloxamer <br /> <br /> nanoparticles with dhs target with percent parasitemia 9.07±0.019 and inhibition <br /> <br /> the growth of parasite was 49.16%. Total RNA isolation and good RNA purity <br /> <br /> were obtained by isolation using TRIzolTM LS Reagent and saponin 0.15% with a <br /> <br /> total RNA obtained 201.28±2.91 and purity of 1.52±0.065. <br />