#TITLE_ALTERNATIVE#
Indian pluceha (Pluchea indica L.) is one of plant which has been used for many years as traditional <br /> <br /> medicine in Indonesia. Indian pluchea leaves are well-known for its ability to deodorize body. <br /> <br /> However, recent studies prove that Indian pluchea le...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/30499 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Indian pluceha (Pluchea indica L.) is one of plant which has been used for many years as traditional <br />
<br />
medicine in Indonesia. Indian pluchea leaves are well-known for its ability to deodorize body. <br />
<br />
However, recent studies prove that Indian pluchea leaves is potentially able to perform as <br />
<br />
antibacterial agent to bacteria infecting skin, such as Propionibacterium acnes and Staphylococcus <br />
<br />
epidermidis. This research was aimed to identify and isolate the compound which contained <br />
<br />
antibacterial activity in Indian pluchea leaves. The crude drug of Indian pluchea leaves was <br />
<br />
extracted by reflux method using increasing polarity solvents, n-hexane, ethyl asetate and ethanol. <br />
<br />
All extracts were monitored using thin layer chromatography. Antibacterial activity of three extract <br />
<br />
were each being tested by using disk diffusion method, microdilution, and bioautography test. <br />
<br />
Based on disk diffusion test, all extracts had inhibiton zones for both of bacteria tests. Microdilution <br />
<br />
test results showed that all extract had MIC value >4096 µg/mL for both bacteria test. <br />
<br />
Bioautography test results indicated that all extract inhibition zone in Propionibacterium acnes and <br />
<br />
not obvious in Staphylococcus epidermidis. Ethanol extract was chosen for fractination as it had the <br />
<br />
highest zone of inhibition in disk diffusion test and clear inhibition zone in bioautography test. Rf <br />
<br />
0,6 used as a guideline for isolating antibacterial compound. Fractionation method was vacuum <br />
<br />
liquid chromatography and purifying method was preparative TLC. The result indicated that one of <br />
<br />
was classified as flavonoid and another could not be identified. Both of isolate had antibacterial <br />
<br />
activity. <br />
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