#TITLE_ALTERNATIVE#

• Main Author: YUNITA EKASARI NIM : 10714014, RIMA
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/30499
• Institution: Institut Teknologi Bandung

Indian pluceha (Pluchea indica L.) is one of plant which has been used for many years as traditional <br /> <br /> medicine in Indonesia. Indian pluchea leaves are well-known for its ability to deodorize body. <br /> <br /> However, recent studies prove that Indian pluchea leaves is potentially able to perform as <br /> <br /> antibacterial agent to bacteria infecting skin, such as Propionibacterium acnes and Staphylococcus <br /> <br /> epidermidis. This research was aimed to identify and isolate the compound which contained <br /> <br /> antibacterial activity in Indian pluchea leaves. The crude drug of Indian pluchea leaves was <br /> <br /> extracted by reflux method using increasing polarity solvents, n-hexane, ethyl asetate and ethanol. <br /> <br /> All extracts were monitored using thin layer chromatography. Antibacterial activity of three extract <br /> <br /> were each being tested by using disk diffusion method, microdilution, and bioautography test. <br /> <br /> Based on disk diffusion test, all extracts had inhibiton zones for both of bacteria tests. Microdilution <br /> <br /> test results showed that all extract had MIC value >4096 µg/mL for both bacteria test. <br /> <br /> Bioautography test results indicated that all extract inhibition zone in Propionibacterium acnes and <br /> <br /> not obvious in Staphylococcus epidermidis. Ethanol extract was chosen for fractination as it had the <br /> <br /> highest zone of inhibition in disk diffusion test and clear inhibition zone in bioautography test. Rf <br /> <br /> 0,6 used as a guideline for isolating antibacterial compound. Fractionation method was vacuum <br /> <br /> liquid chromatography and purifying method was preparative TLC. The result indicated that one of <br /> <br /> was classified as flavonoid and another could not be identified. Both of isolate had antibacterial <br /> <br /> activity. <br />