ISOLATION AND CONVERSION OF PALMITOLEIC ACID FROM CYCLOTELLA STRIATA TBI

• Main Author: WAHYUNI (NIM:10514031), RIRIN
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/30525
• Institution: Institut Teknologi Bandung

One of the tropical marine microalgae that has the potential to produce large amounts of fatty acids is <br /> <br /> Cyclotella striata TBI. This study aimed to isolate palmitoleic acid from C. striata TBI biomass and <br /> <br /> convert palmitoleic acid through metathesis olefin reaction. The research stages included the <br /> <br /> preparation of C. striata TBI biomass in large quantities, isolation and purification of lipids from <br /> <br /> biomass, identification of total fatty acid from lipid C. striata TBI, separation of palmitoleic acid from <br /> <br /> total fatty acid, and conversion of palmitoleic acid through metathesis olefin reaction. The result <br /> <br /> showed that the dry cell mass of C. striata TBI obtained from cultures of 1500 L with an initial <br /> <br /> density of 500000 cells/mL for 8, 9, and 10 consecutive days was 1.474, 1.396, and 0.1393 kg. Dry <br /> <br /> cell of C. striata TBI contains 15% (w/w) lipid. Lipid of C. striata TBI contains 8.8% (w/w) total <br /> <br /> fatty acid which 13.6% (w/w) of its mass is palmitoleic acid. The content of palmitoleic acid isolated <br /> <br /> from total fatty acid with acetone solvent 0.5%, 5%, and 10%, respectively 7.01%, 3.01%, and 0.57%. <br /> <br /> GC-FID chromatogram showed that the peak of palmitoleic acid at 16 min retention time was not <br /> <br /> appeared after olefin metathesis reaction, and a new peak appeared at 32 min retention time. This <br /> <br /> indicates that the conversion of palmitoleic acid occurs.