THE EFFECTS OF TRIS AND HEPES BUFFER SOLUTIONS ON THE ACTIVITY OF Α-AMYLASE FROM BACILLUS AQUIMARIS MKSC 6.2

• Main Author: RACHMAWATI PUTRI (NIM:10512055), SARAH
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/30822
• Institution: Institut Teknologi Bandung

Starch is a polysaccharide molecule conserved in plants. Starch conversion process undergoes three energy intensive and costly steps. α-Amylase belongs to the family of glycoside hydrolase that has the ability to degrade starch molecule. α-Amylase is an endo-acting enzyme that cleaves the α-1,4-glycocide bond in starch granule, producing various kinds of maltooligosaccharides and maltodextrins. Active α-amylase enzymes against starch granule, or commonly known as raw starch degrading enzyme (RSDE), are thought to be able to reduce the energy and cost required in the starch conversion process. One genus of bacteria known as α-amylases producer is Bacillus. α-Amylases derived from Bacillus aquimaris (BaqA) is an RSDE expected to reduce the energy and production cost of starch conversion. This study will observe the characteristics of purified wild type BaqA using Tris buffer pH 7,00 and HEPES buffer pH 7,00. The total concentration of BaqA produced are 27,416 mg/mL and 18,703 mg/mL for Tris and HEPES buffer, respectively. The activities of recombinant BaqA produced are 741,2 U/mL and 161,18 U/mL for HEPES and Tris buffer respectively. While the specific activities are as follows, 27,04 U/mL for HEPES buffer and 8,62 U/mL for Tris buffer