#TITLE_ALTERNATIVE#
Acne is a skin disease can be caused by Propionibacterium acnes and Staphylococcus epidermidis <br /> <br /> bacteria. In recent years, antimicrobial activities of the natural plant materials have been intensively <br /> <br /> explored as an alternative for treating acne, on...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/30981 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Acne is a skin disease can be caused by Propionibacterium acnes and Staphylococcus epidermidis <br />
<br />
bacteria. In recent years, antimicrobial activities of the natural plant materials have been intensively <br />
<br />
explored as an alternative for treating acne, one of that is Nyamplung (Calophyllum inophyllum L.). This <br />
<br />
experiment aims to test the antibacterial activity of ethanol extract from leaves and stem bark of <br />
<br />
Nyamplung against the Propionibacterium acnes and Staphylococcus epidermidis. Extraction was <br />
<br />
carried out by the reflux method using ethanol as a solvent. The selected extract was fractionated using <br />
<br />
liquid-liquid extraction with n-hexane and ethyl acetate as solvents. Diffusion method and <br />
<br />
microdilution were used to test antibacterial activity. From the preliminary test, at a concentration of <br />
<br />
10% ethanol extract of the bark had a largest inhibit zone against Staphylococcus epidermidis with <br />
<br />
diameter inhibition 11.68 ± 0.81 mm. Microdilution test results of ethanol extract, water fraction, and <br />
<br />
ethyl acetate fraction of stem bark had MIC (Minimum Inhibition Concentration) values with the range <br />
<br />
from 1024 - 2048 μg / mL and MBC (Minimum Bactericidal Concentration) values with the range from <br />
<br />
3125 - 6250 μg / mL against Staphylococcus epidermidis, while the n-hexane fraction showed no <br />
<br />
antibacterial activity. The stem bark extract and fractions were tested in bioautography. Bioautography <br />
<br />
showed that the compounds contained in the water fraction with the Rf value of 0.2 on the silica gel <br />
<br />
GF254 TLC plate with the mobile phase of chloroform-ethyl acetate-formic acid (1: 3: 1) gave inhibitory <br />
<br />
areas against S. epidermidis. This compound was proposed to be flavonoid. <br />
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