IMPROVEMENT OF LARVICULTURE PERFORMANCE OF TILAPIA (Oreochromis niloticus) THROUGH SYNBIOTIC FEED CONSISTING OF MICROALGAE PREBIOTICS Nannochloropsis oculata AND Spirulina sp. AND PROBIOTICS Bacillus subtilis

• Main Author: Nafhani Triadi Putri - NIM : 10614037, Talitha
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/31213
• Institution: Institut Teknologi Bandung

<p align="justify">Larviculture is a bottleneck in aquaculture including the cultivation of tilapia (Oreochromis niloticus) . Fish hatchery phase is a critical phase, which happens during the transition of the use of feed inputs from the yolk sac to the feed input from the outside. Constraints that are often encountered in fish hatcheries are low survival caused by a lack of the availability of natural food both qualitatively and quantitatively, as well as the vulnerability of fish diseases caused by pathogenic bacteria. This study aims to develop a synbiotic feed formulation consisting of prebiotics Nannochloropsis oculata and Spirulina sp. and probiotic Bacillus subtilis; and to see its effect on the larviculture performance of tilapia. This research was conducted through 4 stages: (1) formulation and production of synbiotic feed, (2) proximate analysis of synbiotic feed, (3) performance test of synbiotic feed on tilapia post-larvae, and (4) challenge test using 106 CFU/mL Aeromonas hydrophila. During the test, it was conducted measurements of the chemical parameters of water (ammonium, nitrite, nitrate, and phosphate), water physics (temperature, dissolved oxygen , and pH), and total aerobic bacteria testing. The successful formulated synbiotic feed consisted of P10 (Nannochloropsis 10% b/b + Bacillus subtilis 108 CFU/kg), and P5 (Nannochloropsis 5% b/b and Spirulina sp. 5% b/b + Bacillus subtilis 108 CFU/kg). Based on the results of the feed performance test, the largest number of survived fish was found in P5 treatment (72.2%) followed by P10 (62.3%) and control (PK) (42.7%), there were significant differences (p<0.05) in each treatment. The highest increase in average body weight and average body length were found in P5 treatments, (0.118 ± 0.029) gr/ind and (0.69±0.18) cm/ind, respectively. It was followed by P10 with 0.082 ± 0.015 gr/ind and (0.65±0.06) cm/ind while controlled treatment was (0.060 ± 0.004) gr/ind and (0.33±0.036) cm/ind. Based on the value of Food Convertion Ratio (FCR) and Protein Efficiency Ratio (PER) , P5 has the best value, which is 0.91 and 0.541. From the challenge test result, P5 has the highest survival of 83.3% which is significantly different from P10 (70.0%) and PK (56.0%). According to this study, it can be concluded that the synbiotic feed (Nannochloropsis 5% b/b and Spirulina sp. 5% b/b + Bacillus subtilis 108 CFU/kg) can increase survival, growth, and resistance of tilapia post-larvae.<p align="justify">