#TITLE_ALTERNATIVE#

• Main Author: Grace (nim : 11213032), Veronica
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/31463
• Institution: Institut Teknologi Bandung

<br /> <br /> Sweet Potato (Ipomoea batatas L.) are known to produce phenolic compounds that have potential antioxidant properties. Several studies related to in vitro culture as well as analysis of phenol and antioxidant content in sweet potato have been done, but research related to Cilembu Sweet Potato is still very minimal. The aims of this research are to optimize the growth of Cilembu Sweet Potato callus on Murashige and Skoog medium with the addition of BAP and 2,4-D, and to analyze phenol content and antioxidant activity of callus induced from leaves (Kd) and petioles (Kp) of Cilembu Sweet Potato. This research was conducted in three stages. The first stage is the step to optimize the growth of Cilembu Sweet Potato callus. This stage was done on MS solid medium supplemented with BAP with concentration 0; 10 ; and 15 &#61549;M combined with 2,4-D with concentration 0; 0,5; 1; 2,5; and 5 &#61549;M for four weeks. The next stage is the subculture stage which is done based on the results obtained at the previous stage. Callus subculture was conducted during 4 weeks and performed on four types of medium namely medium without BAP and 2,4-D, medium supplemented with 2,4-D 5 &#61549;M without BAP, medium supplemented with BAP 10 &#61549;M without 2,4-D, and medium supplemented with BAP 10 &#61549;M and 2,4-D 5 &#61549;M. The last step in this research is the analysis stage consist of analysis of phenolic content and antioxidant activity. The phenolic content of Cilembu Sweet Potato callus was analyzed using Folin Ciocalteu method and expressed in terms of mg GAE / g dry weight. The determination of anti-radical activity of Cilembu Sweet Potato callus was done by measuring the decrease of 1,1-diphenyl¬-2-picrylhydrazil (DPPH) reagen uptake at 517 nm wavelength using visible spectrophotometry. Best callus growth during 4 weeks of culture was obtained on medium containing 2,4-D 5 &#61549;M only (Kd = 603,33 ± 41,67 mg and Kp = 408,89 ± 28,36 mg). The highest phenolic content were achieved on 2,4-D 5 M and without BAP (Kd = 7,21 &#61617; 0,39 mg GAE / g dry and Kp = 1,26 &#61617; 0,04 mg GAE / g dry weight). The highest antioxidant activity was observed on medium supplemented with BAP 10 &#61549;M without 2,4-D for the callus from leaves explant (1/IC50 Kd = 2,57 &#61617; 0,31 L/g). Meanwhile, the highest antioxidant activity of callus from petioles explant was induced from medium containing 2,4-D 5 &#61549;M without BAP (1/IC50 Kp = 1,06 &#61617; 0,04 L/g). Thus, it can be concluded that the leaves and petiolus of Cilembu Sweet Potato can be used as explants for callus induction. In addition, phenolic compounds which has the potential as antioxidants can be produced from Cilembu Sweet Potato callus through in vitro culture. <br />