#TITLE_ALTERNATIVE#

• Main Author: PUTRI DEWIANTI NIM: 20716013, ZENITH
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/31902
• Institution: Institut Teknologi Bandung

Inflammation plays an important role in cancer, atherosclerosis and ischemic heart <br /> <br /> disease, as well as several neurodegenerative diseases which are also the leading <br /> <br /> cause of death in the world. Pomegranate (Punica granatum L.) has been used for a <br /> <br /> long time to treat various diseases, including inflammation, which is expected to be an <br /> <br /> alternative therapy. Screening the pharmacological activities of active ingredients and <br /> <br /> knowing the mechanism of its interaction with the target protein is an expensive <br /> <br /> process and requires a long time if only done using experimental animals. So this study <br /> <br /> aims to predict the interaction of compounds contained in pomegranate peels (Punica <br /> <br /> granatum L) against cyclooxygenase in in silico, test the toxicity of water fraction, and <br /> <br /> also test the anti-inflammatory of ethanol extract and water fraction in zebrafish <br /> <br /> embryos. Interactions are predicted by docking to cyclooxygenase-1 (PDB ID: 5WBE) <br /> <br /> and cyclooxygenase-2 (PDB ID: 5IKR). Toxicity test was carried out based on OECD <br /> <br /> number 236, while the anti-inflammatory test of zebrafish embryos was carried out by <br /> <br /> staining using neutral red at five different concentrations induced by carrageenan. The <br /> <br /> best compounds by docking are punicic acid and kaempferol, have a better interaction <br /> <br /> than native ligands with binding energy -7.7619, -7.3755 at 5WBE and -8.3216, - <br /> <br /> 7.1262 at 5IKR, respectively. Lethal Concentration 50 (LC50) of water fraction is <br /> <br /> 236.57 ± 6.1 µg / mL, categorized as safe according to the environmental safety <br /> <br /> category of the OECD. Whereas the results of anti-inflammatory testing are observed <br /> <br /> based on fluoroscence intensity, survival rate, and heart beat rate, with the best <br /> <br /> concentration result on ethanol extract is 23.6 µg/mL and water fraction is 21.6 µg/mL. <br /> <br /> So it can be concluded that ethanol extract and water fraction of pomegranate peels <br /> <br /> have the potential as candidates for anti-inflammatory drugs. <br />