EVALUATION OF RADIATION EXPOSURE EFFECT AGAINST CYTOTOXIC AND GENOTOXIC EFFET TO Allium cepa AS BIOINDICATOR OF OCCUPATIONAL ENVIRONMENT CONDITION IN THE HOSPITAL RADIOLOGIC UNIT

• Main Author: Sopandi, Yunika
• Format: Theses
• Language: Indonesia
• Subjects: Teknik saniter dan perkotaan; teknik perlindungan lingkungan
• Online Access: https://digilib.itb.ac.id/gdl/view/32111
• Institution: Institut Teknologi Bandung

Abstract: The application of non surgical diagnostic procedure such as fluoroscopy and interventional cardiology produce high radiation dose to patient and the medical staff as well. It is important to monitor the condition of occupational environment due to ionizing radiation exposure in order to protect the worker from the long-term effect due to radiation exposure. The monitoring of direct effect to organism due to ionizing radiation exposure is still seldom to be observed. This research is conducted to evaluate the occupational environment condition of the hospital that is exposed by ionizing radiation, using Allium cepa as bio-indicator. This research is carried out in RSUP dr. Hasan Sadikin Bandung in the four (4) different sites selected, examination and operator room in interventional cardiology and fluoroscopy procedure, operator room in CT-Scan and radiotherapy procedure. The effects observed on the A. cepa are mitotic index and chromosome aberration. The calculation result of radiation dose to all operator room in the four study site remained below the threshold value, that is 20 mSv/year, as well as the mean value of the mitotic index and chromosome aberration that can be said is not toxic to A. cepa compare to the negative control group. While the accumulation dose of radiation in interventional cardiology examination room is above the threshold, it is in accordance with the value of mitotic index and chromosome aberration resulted. Based on the dose-response curve, the relationship between accumulated dose and the percentage of mitotic index as well as chromosome aberration is logarithmic, with high determination value (R2) respectively 0.862 and 0.933.