Carbon Paste Electrode Modified Molucelarly Imprinted Poly(3-aminophenol) for Voltammetric Determination of Uric Acid
Uric acid was a major product of purine metabolisme in the human body that mostly excreted through urine and in very small portion through sweat. Deficiency or excess levels of uric acid indicates some diseases such as gout, hyperuricemia, Leysh-Nyan disease, obesity, diabetes, high blood pressur...
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| Format: | Final Project |
| Language: | Indonesia |
| Subjects: | |
| Online Access: | https://digilib.itb.ac.id/gdl/view/32222 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Uric acid was a major product of purine metabolisme in the human body that mostly
excreted through urine and in very small portion through sweat. Deficiency or excess
levels of uric acid indicates some diseases such as gout, hyperuricemia, Leysh-Nyan
disease, obesity, diabetes, high blood pressure, high cholesterol, liver disease, and
kidney. Analysis of uric acid levels in urine can be performed using voltammetry
method. In this study, the working electrode used was Carbon Paste Electrodes (CPE)
modified with molecularly imprinted poly (3-aminophenol) (MIP). Carbon paste
modified with MIP was made using cyclic voltammetry (CV) technique in solution
mixture of 20 mM 3-aminophenol monomer, 10 mM uric aid 0,1 M NaOH, in
potential range of -0,2 V - 1 V, and scan rate of 100 mV / s. 0,1 M phosphate buffer
pH 7 was used as supporting electrolyte for uric acid determination. Calibration
curve was linear at concentrations 10-500 ?m with a detection limit of 3,15 ?M.
Determination of uric acid sample in urine samples using the electrode showed good
accuracy. |
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