Quantitative Analysis of Phenol, 2,4-Dinitrophenol, and 4-Aminophenol by High Performance Liquid Chromatography (HPLC)
The analysis of phenolic compound can be conducted by using various methods, for examples spectrophotometry and chromatography. In spectrophotometry, the separation of the compounds was difficult because the absorbance was obtained from the total compounds which are influencing each other. In some p...
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| Format: | Final Project |
| Language: | Indonesia |
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| Online Access: | https://digilib.itb.ac.id/gdl/view/32279 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | The analysis of phenolic compound can be conducted by using various methods, for examples spectrophotometry and chromatography. In spectrophotometry, the separation of the compounds was difficult because the absorbance was obtained from the total compounds which are influencing each other. In some previous studies, this kind of analysis were not further explored especially in 4-aminophenol compound which has lower sensitivity than phenol and 2,4-dinitrophenol compounds. The analysis of phenol, 2,4-dinitrophenol, and 4-aminophenol has been conducted by using High Performance Liquid Chromatography (HPLC). The detector used in this analysis was UV, the column was LiChrospher® RP-18EC which was non-polar, and the eluen was methanol : water (4:1). The wavelength determination in this analysis was based on each maximum absorption of phenol, 2,4-dinitrophenol, and 4-aminophenol obtained from the maximum absorption experiment using UV spectrophotometer. The result shows that phenol exhibits the maximum absorbance at 273 nm, 2,4-dinitrophenol exhibits the maximum absorbance at 356 nm, and 4-aminophenol exhibits the maximum absorbance at 405 nm. In this analysis, the influence of phenol and 2,4-dinitrophenol toward the sensitivity of 4-aminophenol was also investigated. Based on the analysis using HPLC applied in various compounds with various elements, it show that 4-aminophenol in fact has the lowest sensitivity yet has the potential to be separated at the retention time which is not owned by phenol and 2,4-dinitrophenol. In this case, phenol and 2,4-dinitrophenol influence the sensitivity of 4-aminophenol in compounds with various elements. Therefore, it can be concluded that phenol and 2,4-dinitrophenol can affect 4-aminophenol. |
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