Degradation of Used Tissue Papers Using Recombinant Endo-1,4-?-glukanase

Used tissue papers are household waste containing high cellulose. Cellulose from such used tissue paper waste can be degraded to free sugar products that can be used for multiple purposes. In the present study, the degradation of used tissue paper with various brands using the recombinant endo-1,...

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Main Author: Rizki Noviandi, Tri
Format: Final Project
Language:Indonesia
Subjects:
Online Access:https://digilib.itb.ac.id/gdl/view/32475
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Institution: Institut Teknologi Bandung
Language: Indonesia
id id-itb.:32475
spelling id-itb.:324752018-12-20T11:31:44ZDegradation of Used Tissue Papers Using Recombinant Endo-1,4-?-glukanase Rizki Noviandi, Tri Kimia Indonesia Final Project recombinant endo-1,4-?-glucanase, tissue paper waste cellulose, temperature INSTITUT TEKNOLOGI BANDUNG https://digilib.itb.ac.id/gdl/view/32475 Used tissue papers are household waste containing high cellulose. Cellulose from such used tissue paper waste can be degraded to free sugar products that can be used for multiple purposes. In the present study, the degradation of used tissue paper with various brands using the recombinant endo-1,4-?-glucanase was investigated. Firstly, enzyme from recombinant Bacillus megaterium induced by xylosa was isolated. Secondly, used tissue papers were hydrolyzed by the recombinant endo-1,4-?-glucanase at three different variations of temperature. Finally, reducing sugar products were determined by the DNS method. The experimental results showed that used tissue paper waste (10 mg) from all various brands hydrolyzed by recombinant endo-1,4-?-glucanase at 50 oC for 5 days released 0.0007 mmol reducing sugar. Thus, it can be concluded that recombinant endo-1,4-?-glucanase can be used to hydrolyzed used tissue paper. text
institution Institut Teknologi Bandung
building Institut Teknologi Bandung Library
continent Asia
country Indonesia
Indonesia
content_provider Institut Teknologi Bandung
collection Digital ITB
language Indonesia
topic Kimia
spellingShingle Kimia
Rizki Noviandi, Tri
Degradation of Used Tissue Papers Using Recombinant Endo-1,4-?-glukanase
description Used tissue papers are household waste containing high cellulose. Cellulose from such used tissue paper waste can be degraded to free sugar products that can be used for multiple purposes. In the present study, the degradation of used tissue paper with various brands using the recombinant endo-1,4-?-glucanase was investigated. Firstly, enzyme from recombinant Bacillus megaterium induced by xylosa was isolated. Secondly, used tissue papers were hydrolyzed by the recombinant endo-1,4-?-glucanase at three different variations of temperature. Finally, reducing sugar products were determined by the DNS method. The experimental results showed that used tissue paper waste (10 mg) from all various brands hydrolyzed by recombinant endo-1,4-?-glucanase at 50 oC for 5 days released 0.0007 mmol reducing sugar. Thus, it can be concluded that recombinant endo-1,4-?-glucanase can be used to hydrolyzed used tissue paper.
format Final Project
author Rizki Noviandi, Tri
author_facet Rizki Noviandi, Tri
author_sort Rizki Noviandi, Tri
title Degradation of Used Tissue Papers Using Recombinant Endo-1,4-?-glukanase
title_short Degradation of Used Tissue Papers Using Recombinant Endo-1,4-?-glukanase
title_full Degradation of Used Tissue Papers Using Recombinant Endo-1,4-?-glukanase
title_fullStr Degradation of Used Tissue Papers Using Recombinant Endo-1,4-?-glukanase
title_full_unstemmed Degradation of Used Tissue Papers Using Recombinant Endo-1,4-?-glukanase
title_sort degradation of used tissue papers using recombinant endo-1,4-?-glukanase
url https://digilib.itb.ac.id/gdl/view/32475
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