IDENTIFICATION OF POTENTIAL BACTERIA AND ENZYMES DEGRADING MACROALGAE SARGASSUM SP CELL WALL

• Main Author: Nurfitriani
• Format: Final Project
• Language: Indonesia
• Subjects: Kimia
• Online Access: https://digilib.itb.ac.id/gdl/view/34582
• Institution: Institut Teknologi Bandung

Macroalgae have various benefits in food, health, industry and bio-energy. Mechanical and chemical extraction methods are still used in order to obtain chemical compounds and useful metabolites from macroalgae. One of more practical and efficient method is enzymatic method. The aims of this research to identify the bacteria which can produce degradative enzymes of macroalgae cell wall and to determine the activity of several produced enzymes. The research was conducted by growing Sargassum sp symbiotic bacteria, isolating the bacteria, screening the bacteria that produced cellulase, xylanase and alginate lyase by using spesific subtrates in each media. Xylanase and cellulase activity were conducted using 3,5- dinitrosalicylic (DNS) method. The identity of the isolated bacteria were conducted using rybotyping analysis, which consist of chromosomal DNA isolation, 16s rRNA gene isolation using Polymerase Chain Reaction (PCR) method and determining their phylogenetic tree. The result showed that there are 5 isolates, namely P1 to P5 that produce xylanase. P4 and P5 also produce cellulase, while P1, P2 and P5 produce alginate lyase. The specific activity of xylanase activity of P1, P2, P3, P4, and P5 respectively were 0,15 U/µg, 0,217 U/µg, 0,262 U/µg, 0,356 U/µg dan 0,293 U/µg The spesific activity of cellulase activity of P4 was 865 U/µg, and P5 was 8,55 U/µg. The result of ribotyping analysis showed that bacteria P2 had 99% sequence similarity with Pseudomonas xanthomarina strain KMM 1447, bacteria P3 was Staphylococcus saprophyticus subsp. bovis strain GTC 843, bacteri P4 had 99% sequence similarity with Pseudomonas balearica strain SP1402, and bacteria P5 was Pseudomonas stutzeri ATCC 17588 = LMG 11199 strain ATCC 17588.