STUDY SEPARATION OF STARCH AND MALTOSE BY SIZE EXCLUSION CHROMATOGRAPHY USING SEPARATOR MATERIAL CONSIST OF CELLULOSE ACETATE AND CHITOSAN

• Main Author: Samsiar
• Format: Theses
• Language: Indonesia
• Subjects: Kimia
• Online Access: https://digilib.itb.ac.id/gdl/view/34994
• Institution: Institut Teknologi Bandung

Program) Low calories sweetener could be produced from maltose. Maltose and starch are dimer and polymer of glucose, respectively. Starch is divided into two types, namely amylopectin and amylose. Amylopectin is composed by glucose units, bound via 1,4-and 1,6-glycosidic linkages, whereas amylose is composed by glucose units bound via 1,4-glycosidic linkages. Maltose could be produced from starch hydrolysis process with the mediation of enzymes, acids or peroxides. Separation of starch and maltose is one of the alternative ways to increase the efficiency of enzymatic hidrolysis of starch to maltose. In this research, a study of separation of starch and maltose by size exclusion chromatography using separator materials consist of cellulose acetate and chitosan having a ratio of 14 : 1. Cellulose acetate is one of the polymers that is widely used as porous material. Bibliographical survey informs that cellulose acetate could also be mixed with another compounds to form polymer blends. The aim of such approach is to improve the properties of cellulose acetate materials. In this research, cellulose acetate was mixed with chitosan. The purpose of mixing cellulose acetate and chitosan is to obtain more porous materials for a better performance in separation. The obtained materials could separate a mixture of starch and maltose. Starch could be analyzed quantitatively using spectrophotometric method with I2/KI rezatt. Starch reacts with I3- to form a blue compound having maximum absorption at a wavelength of 600 nm. Spectrophotometric method could also be applied for quantitative analysis of maltose. Maltose reacts with 3,5- dinitrosalicylic acid reagent to form compounds, it was having a maximum absorption at a wavelength of 540 nm. In this research, it was found that the presence of starch did not interfere with the measurement of maltose, and vice versa. This research also was conducted to study the effect of shape variations of the separator material in the form of beads and powder, as well as the effect of the addition of crosslinking agent, namely succinic acid and glutarat acid, on material's ability to separate a mixture of starch and maltose. The results showed that the form variation of filler material of chromatography columns as well as types variation crosslinking agent lead to differences in the separation profile. The addition of 0,30 gram of succinic acid as crosslinking agent gave improvement in separation performance. It was observed that the maximum concentration of starch and maltose in the samples was obtained in a longer interval of fractionation time. Thus, the separation performance increased. Material in the form of powder gives the improvement of separation performance. It was observed that the maximum concentration of starch and maltose in the sample retained in a longer interval of fractionation time, compared to the particle in the form of beads. It can be concluded that the material is capable of separating the starch and maltose on taking the most using 0.30 grams of succinic acid to column filling material in the form of powder. Separation of starch and maltose was concluded occur as a consequence of pore parameters of the separator material. SEM photographs show the existence of pores on the surface of the separator material. EDX spectra showed no significant effect of the addition of succinic acid in the separator material. In addition, the IR spectra reveals the interaction between cellulose acetate, chitosan and crosslinking in the separator material that play a role in forming the pore. IR spectra showed that between cellulose acetate, chitosan,and carboxylic acid there is ester bond.