THE EFFECT OF RICE AROMATIC GALANGAL HERB AND CHOLERA TOXIN B SUBUNIT (CTB) ON MICE Mus musuclus C57BL/6 AS ANIMAL MODELS INDUCED INFLAMMATORY BOWEL DISEASE

• Main Author: Nurul Ilmi, Annisa
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/36088
• Institution: Institut Teknologi Bandung

Inflammatory Bowel Disease (IBD) is a disease caused by dysregulation of immune system towards guts microbiota. Ulcerative Colitis (UC), one of IBD is characterized by an increase of pro-inflammatory cytokine secretion which can develop into colorectal cancer. Currently, the treatment of IBD still uses anti-inflammatory medicine which comes with expensive cost. Rice Aromatic Galangal Herb or Jamu Beras Kencur (JBK) and Cholera toxin B subunit (CTB) have an anti-inflammatory effect. It is necessary to develop an anti-inflammatory drug from both compounds. The aim of this research was to identify the potential of JBK combined with CTB as anti-inflammatory of C57BL/6 Mice 6-8 weeks old as animal models. The anti-inflammatory activity carried out in mice that exposed Dextran Sulfate Sodium (DSS) 3,5% orally for 12 days, while drinking water-given group served as control. The mice were divided into 5 groups of DSS treatment and 5 groups of healthy, the treatment group was given Phosphate Buffer Saline (PBS), JBK at 0,037 gr/kg, CTB at 10 µg, and dual (combination of JBK and CTB). Dexamethasone 0,5 mg/kg was used in DSS treatment group as positive control, meanwhile healthy mice group used sugar solution at 0,0153 gr/kg. Observation on body weight and Disease Activity Index (DAI) were conducted daily. At the end of the test, mice were sacrified and their organs were isolated. Their organs were weighed for organ index determination. The entire colon length was measured. The colon was prepared for histopathological analysis using Hematoxylin Eosin (HE) staining and gene expression mRNA level analysis. The inflammation related gene expression Tumor Necrosis Factor Alpha (TNF-?) and Transforming Growth Factor Beta (TGF-?) was carried using Real Time PCR method. Combination of JBK and CTB has recovery effect. This anti-inflammation activity shown by the decrease of DAI score and reduced body weight loss better than mice with DSS+JBK and DSS+CTB. There were no significant differences between DSS and non-DSS treatment group (P>0,05) based on organ index. It means that the test was safe and has no effect on mice organ (heart, liver, spleen, and kidney). This recovery activity on DSS+Dual was shown by the stabil colon length at 6,5±0,1 cm compared with healthy mice group and significantly different to DSS and PBS combination at 4,2±0,4 cm (P<0,05). DSS+JBK, DSS+CTB, and DSS+Dual treatment group shown the wound recovery activity by decreased histopathological scores were better than DSS+Dexamethasone treatment group, as. This recovery activity shown by decreased of crypts ulceration, mucosa lesion and epitel erosion. Results showed that TNF-? expression was highest in DSS+CTB group 58 fold and DSS+Dual group at 29,91 fold on IBD mice demonstrate that JBK and CTB combination played a role to enhance colon repair through TNF-? released towards intestinal stem cell. TNF-? expression was low in DSS+Dual group at the end of solution addition, showing that mucosa repair was faster in recovery period. Altogether, these results has shown combination of JBK and CTB has potential as anti-inflammatory functional drink to animal model.