ANALYTICAL METHOD DEVELOPMENT FOR DIPHENHYDRAMINE HCL USING MOLECULARLY IMPRINTED POLYMER SOLID PHASE EXTRACTION (MIP-SPE) BY SPECTROPHOTOMETRY UV-VIS

• Main Author: Ismail, Faisal
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/36615
• Institution: Institut Teknologi Bandung

Molecularly Imprinted Polymer (MIP) is a polymer made by molecular imprinting techniques with a good affinity to the template molecule, so that MIP has a highselectivity and sensitivity for the template recognition. The purpose of this study wasto synthesize selective Molecularly Imprinted Polymer (MIP) for separation diphenhydramine and validation analysis methods using spectrophotometry. Synthesis of MIP was prepared by using diphenhydramine as the molecular template,Metacrylic acid (MAA) as the functional monomer, ethylenglycolol dimethacrylate(EGDMA) as a crosslinker, and 2.2'- azobisisobutironitrile (AIBN) as the initiator and chloroform as the porogenic solvent. Imprinting Factor (IF) was calculated by comparing the area under the curve of diphenhydramine on the imprinted polymer with a comparable nonimprinted polymer (NIP). MIP was synthetized used bulk polymerization with a ratio of template molecules, functional monomers, crosslinker (1: 4: 20) with resulted. IF valve of 1.85. MIP was characterized by FourierTransform Infrared (FTIR) and evaluated using equilibrium batch rebinding method.Selectivity test showed that MIP can be used for selective extraction of diphenhydramine. Analysis method of diphenhydramine with spectrophotometry showed valid result, linearity with R2 = 0.9996, accuracy with recovery of 99,13 -100.65%, and precision with coefficient of variance 0.71%.