Analisis Sequence Related Amplified Polymorphism (SRAP) untuk Keanekaragaman Genetika Brotowali (Tinospora crispa) dari Enam Provinsi di Indonesia
Indonesia is a tropical region that has great potential as a mega biodiversity. At least 1,000 species are used as medicinal plants, one of which is brotowali (Tinospora crispa). Brotowali is believed to be a reliever for fever, hypertension, and others. Data inventory in the form of chemical com...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/39906 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Indonesia is a tropical region that has great potential as a mega biodiversity. At
least 1,000 species are used as medicinal plants, one of which is brotowali
(Tinospora crispa). Brotowali is believed to be a reliever for fever, hypertension,
and others. Data inventory in the form of chemical compounds, utilization of local
wisdom-based medicinal plants, and genetic diversity to prevent biopiracy need to
be done. This study aims to identify brotowali genetic diversity from six
Indonesian provinces with a molecular marker Sequence-Related Amplified
Polymorphism (SRAP). Samples were obtained from the Balai Besar Penelitian
dan Pengembangan Tanaman Obat dan Obat Tradisional (B2P2TOOT), Health
Research and Development Agency, Ministry of Health as many as 46 individuals
in the form of dried leaves originating from East Java, West Kalimantan, East
Kalimantan, Central Kalimantan, North Kalimantan, and NTB. Amplification of
DNA fragments was carried out using three primer pairs (Me1-Em1, Me3-Em1,
and Me4-Em1) based on the results of primer selection. Data analysis includes
analysis of intrapopulation, interpopulation and cluster analysis. 35 bands with a
size range of 100-1400 were obtained from the SRAP PCR results. The analysis
showed the number of alleles observed (na) = 2 ± 0.0 alleles, effective number of
alleles (ne) = 1.127 ± 0.171, number of polymorphic loci (A) = 37 loci, Shannon
index information (I) = 0.183 ± 0.157, heterozygosity (H) = 0.097 ± 0.106, and
the percentage of polymorphic loci (PPL) = 100%. The results of the dendogram
tree using the Unweighted Pair-Group Method (UPGMA) and Principal
Component Analysis (PCA) methods explain the existence of grouping patterns
and observed NTB apart from other provinces. The conclusion of this study shows
that SRAP molecular markers can be used to identify the genetic diversity of
brotowali (T. crispa) from six provinces in Indonesia at the species level.
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