Analisis Sequence Related Amplified Polymorphism (SRAP) untuk Keanekaragaman Genetika Brotowali (Tinospora crispa) dari Enam Provinsi di Indonesia

• Main Author: Andriyani Laela, Pradita
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/39906
• Institution: Institut Teknologi Bandung

Indonesia is a tropical region that has great potential as a mega biodiversity. At least 1,000 species are used as medicinal plants, one of which is brotowali (Tinospora crispa). Brotowali is believed to be a reliever for fever, hypertension, and others. Data inventory in the form of chemical compounds, utilization of local wisdom-based medicinal plants, and genetic diversity to prevent biopiracy need to be done. This study aims to identify brotowali genetic diversity from six Indonesian provinces with a molecular marker Sequence-Related Amplified Polymorphism (SRAP). Samples were obtained from the Balai Besar Penelitian dan Pengembangan Tanaman Obat dan Obat Tradisional (B2P2TOOT), Health Research and Development Agency, Ministry of Health as many as 46 individuals in the form of dried leaves originating from East Java, West Kalimantan, East Kalimantan, Central Kalimantan, North Kalimantan, and NTB. Amplification of DNA fragments was carried out using three primer pairs (Me1-Em1, Me3-Em1, and Me4-Em1) based on the results of primer selection. Data analysis includes analysis of intrapopulation, interpopulation and cluster analysis. 35 bands with a size range of 100-1400 were obtained from the SRAP PCR results. The analysis showed the number of alleles observed (na) = 2 ± 0.0 alleles, effective number of alleles (ne) = 1.127 ± 0.171, number of polymorphic loci (A) = 37 loci, Shannon index information (I) = 0.183 ± 0.157, heterozygosity (H) = 0.097 ± 0.106, and the percentage of polymorphic loci (PPL) = 100%. The results of the dendogram tree using the Unweighted Pair-Group Method (UPGMA) and Principal Component Analysis (PCA) methods explain the existence of grouping patterns and observed NTB apart from other provinces. The conclusion of this study shows that SRAP molecular markers can be used to identify the genetic diversity of brotowali (T. crispa) from six provinces in Indonesia at the species level.