METHOD DEVELOPMENT FOR DETERMINATION OF ALUMINIUM IN BAKSO FOOD PRODUCTS USING VISIBLE SPECTROPHOTOMETRY
Since aluminium is a toxic metal and aluminium compound is suspected still to be illegally added into food product, analytical method for determination of aluminium in this food products is needed. Commonly, quantitative analysis of aluminium is based on spectroscopic methods such as Atomic Absor...
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| Format: | Theses |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/40126 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Since aluminium is a toxic metal and aluminium compound is suspected still to be
illegally added into food product, analytical method for determination of aluminium
in this food products is needed. Commonly, quantitative analysis of aluminium is
based on spectroscopic methods such as Atomic Absorption Spectroscopy (AAS)
and Induced Coupled Plasma (ICP). However, these instruments are not always
available in any testing laboratories and hence alternative method executable
applying simpler equipment is needed. The aim of this research was to obtain an
analytical method for determination of aluminium in food using visible
spectrophotometry as an alternative method. Aluminium was reacted with Alizarin
Red S to form colored complex compound measurable in the visible light region.
The optimum pH for the complex formation was around 5 with a maximum
absorption wavelength of 494 nm. The calibration curve in the concentration range
of 4-14 ppm showed good linearity with the equation of y = 0.0295x + 0.3287 and
the correlation coefficient (r) of 0.99. Absorbance of the complex measured at 494
nm was stable up to 70 minutes. Furthermore complex formation under the
influence of disturbing metal cations such as Pb
2+
, Cu
2+
, Fe
2+
, Fe
3+
, and Zn
2+
was
observed. Prior measurement, food samples need to be prepared by ashing method.
The precision of the method is calculated as relative standard deviation (RSD) of
intra- and inter- days with the values of 2.11 and 2.31% respectively, while
accuracy calculated as percent recovery at concentrations of 40, 50, and 60 ppm
were 82.07, 84.87, and 93.88%. Limits of detection (LOD) and limits of
quantification (LOQ) calculated statistically were 2.04 and 3.74 ppm, respectively.
Based on the overall results, it can be concluded that the method developed can be
used as an alternative method for determination of aluminum in food samples.
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