ISOLASI DAN UJI AKTIVITAS ANTIMIKROBA JAMUR ENDOFIT YANG TERASOSIASI DENGAN LAMUN (SEAGRASS) DARI PERAIRAN PULAU KALIAGE KECIL KEPULAUAN SERIBU

• Main Author: Afchohi Burhan, Muhammad
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/40199
• Institution: Institut Teknologi Bandung

Increased cases of antibiotic resistance bacteria has encouraged the search for new antibiotic alternatives. There are many natural sources that have the potential to produce secondary metabolites that are bioactive in nature, one of which is endophytic microbes.This study aims to isolate endophytic fungi that are associated with seagrass originating from Pulau Kaliage Kecil Kepulauan Seribu and investigate their antimicrobial activity against Escherichia coli ATCC 8739 and Staphylococcus aureus ATCC 6538 accompanied by determinating MIC and MBC values. This study consisted of taking seagrass samples then continued with the isolation of endophytic fungi and fermented. The results of this research were obtained 15 fungal isolates that were coded as FKAK C1 to FKAK C15. Then the mycelium was separated from the culture medium by centrifugation and then extracted by maceration method while the culture media was extracted by liquid-liquid extraction method using ethyl acetate solvents. Each extract obtained from mycelium and culture media was tested for antimicrobial activity by disc diffusion method and then determined the minimum inhibitory concentration (MIC) value with microdilution method and minimum bactericidal concentration (MBC) with growth methods on agar media. The greatest antibacterial activity was showed by mycelium culture of FKAK C5 extract (64 µg/ mL), mycelium culture and culture media of FKAK C6 extract (64 µg/mL) and mycelium culture of FKAK C11 extract (64 µg/ mL) against Staphylococcus aureus and extract of culture media FKAK C11 (32 µg/mL) against Escherichia coli. The greatest antifungal activity was also showed by FKAK C11 mycelium culture of 64 ?g /mL extract. The lowest MBC value was 64 µg /mL obtained from FKAK C11 mycelium culture extract against Staphylococcus aureus and extract of culture media FKAK C11 (128 µg /mL) against Escherichia coli. While the lowest MFC value was 64 ?g /mL for Candida albicans obtained from FKAK C11 mycelium culture extract.