ENROFLOXACIN RESIDU ASSAY IN CHICKEN AND EGGS USING MOLECULARLY IMPRINTED-SOLID PHASE EXTRACTION AND UV-VIS HPLC

• Main Author: Rohana, Evieta
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/40272
• Institution: Institut Teknologi Bandung

Enrofloxacin is fluoroquinolone antimicrobial agent which is widely used in poultry. Uncontrolled application in poultry can accelerate the anti microbial resistency. It is saved in muscle tissue, skin and fat, liver and kidney. Biological sample has complex matrix. It need both complex and complicated preparation to produce fast and accurate result. Nowadays, the method of separation using Molecular Imprinted Polymer (MIP) is developed to solve the analytical problem caused complex matrix. MIP is a polymer that made using templating method the molecule that has affinity with template so it can elevate the selectivity and sensitivity. In this study, MIP was made using enrofloxacin as template molecule, itaconic acid as monomer, etilenglikol dimetakrilat (EGDMA) as crosslinker, azobisisobutironitril (AIBN) as initiator, chloroform: methanol (4:1) as porogen. Synthesis MIP did through exaggerate polimeration using molecule ratio of template:monomer:crosslinker 1:6:20. It produced 1,84 as an IF. MIP that produced was selective for enrofloxacine. Enrofloxacine assay using HPLC (UV-Vis detector), C18 coloumn and 20 mM phosporic acid pH 3,0: acetonitrile (83:17) as mobile phase. It produced R2 0,9990 as linearity test result, recovery between 98,75%-101,84% as the result of accuracy test and RSD 1,83% as result of precision test. LOD of this method was 1,23 µg/ml and LOQ 4,09 µg/ml. Molecularly Imprinted Solid Phase Extraction (MISPE) method that appicated on chicken muscle that added by enrofloxacine produced 8,83±15,19 mg/kg and did not detected on eggs.