AKTIVITAS ANTIOKSIDAN EKSTRAK AKAR, RANTING, DAN DAUN KELOR (MORINGA OLEIFERA LAMK.) DENGAN METODE DPPH DAN ABTS
Free radicals are compounds that have unpaired electrons and are reactive so they can attack to bind to molecular electrons in the surrounding environment. The adverse effects of free radicals can be reduced by antioxidant. Antioxidant can inhibit oxidation reactions of free radicals. Antioxidants...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/40515 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Free radicals are compounds that have unpaired electrons and are reactive so they can attack to bind to
molecular electrons in the surrounding environment. The adverse effects of free radicals can be reduced
by antioxidant. Antioxidant can inhibit oxidation reactions of free radicals. Antioxidants can be found in
various plants, such as Moringa oleifera L. This study aimed to examine the antioxidant activity of roots,
branches, and leaves of kelor using DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS [2,2'-azino-bis (3-
ethylbenzothiazoline-6 -sulphonic acid)] methods; determine total phenol and flavonoid; analyze the
correlation between total phenol, flavonoid with IC50 DPPH and IC50 ABTS, and analyze the correlation of
DPPH and ABTS methods in the test sample. Extraction was carried out using the reflux method using
three kinds of solvents with increasing polarity. Determination of IC50 DPPH and IC50 ABTS, total phenol
and total flavonoid was done using ultraviolet-visible spectrophotometry. Correlation between total
phenol, flavonoid with IC50 DPPH and ABTS, and correlation between two methods was performed by
Pearson’s method. Ethanol extract of parts of kelor gave IC50 DPPH in the range of 51.87 –79.32 µg/mL,
meanwhile IC50 ABTS 44.70–92.04 µg/mL. Ethanol branches extract of kelor showed the highest total
phenolic content (8.17 g GAE/100 g) and ethanol leaves extract of kelor had the highest flavonoid
content (34.93 g QE/100 g). Total phenolic content in branches extract correlated negatively significant
to IC50 DPPH and ABTS. IC50 DPPH of all samples correlated positively significant with IC50 ABTS. Ethanol
extract of roots, branches and leaves of kelor were strong antioxidant by DPPH and ABTS methods.
Phenolic compounds were main contributor in antioxidant activity of branches extract by DPPH and
ABTS methods. DPPH and ABTS methods gave linear results in antioxidant activities of all samples.
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