ALLERGENICITY OF RAW SHRIMP AND DRIED SHRIMP PRODUCT ON MALE GUINEA PIG

• Main Author: Prabawanti, Dian
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/44346
• Institution: Institut Teknologi Bandung

Type I hypersensitivity reaction is mediated by IgE antibody, when sensitised individual’s exposed to allergen, allergen will react with IgE on the surface of mast cell and basophil, releasing mediators responsible for the allergic reaction. Food allergy is one type of allergy found in community and shrimp is one type of food that is known to be responsible for some allergic reactions. Sensitisation to food could occur through consumption of food, contact related to occupation, or through sensitisation by cross-reacting allergen. Food is usually processed before it’s consumed and processing it might alter its allergenicity. This research aims to test the ability of raw shrimp and dried shrimp (ebi) to sensitise and to elicite allergic reaction through the active ii cutaneous anaphylaxis test. Guinea pig was used in the research. Guinea pigs were randomly placed in the test groups which consisted of one normal group and three sensitisation groups (raw shrimp extract 25% 0,8mL, home made dried shrimp extract 6,08% 0,8mL, and commercial ebi extract 6,08% 0,8mL. The groups are challenged on the 21 sensitisation by intradermal injection. Wheals area and redness score were used as parameter. Statistical significance of redness score were found since 15 minutes after injection. Statistical significance of wheals between allergic and normal groups for the challenge using raw shrimp extract and commercial ebi extract were found at 30, 45, 60, 75, 90, 120, 135, and 150 minutes after injection, meanwhile challenge using home made dried shrimp extract gave statistical significance at 30, 60, 75, 90, 105, 120, 135, and 150 minutes after injection. Allergic group not only gave significant result to the extract they are sensitized with but also to the other extract used in this research. Raw shrimp extract gave the biggest value of wheal area among others.