ISOLASI SENYAWA IDENTITAS DARI DAUN KELOR (MORINGA OLEIFERALAMK., MORINGACEAE)
Marker compounds are specific compounds present in plant with a high amount or are the dominan compound. The marker compound may be used as a reference in the determination of the content of an extract or used in the confirmation identity of a plant and standardization for herbal medicine. Moring...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/44368 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Marker compounds are specific compounds present in plant with a high amount or are the
dominan compound. The marker compound may be used as a reference in the determination of
the content of an extract or used in the confirmation identity of a plant and standardization for
herbal medicine. Moringa oleifera Lamk is one of the plants which used by community with
many pharmacological activities. Plant part of moringa plants that are often used and has
therapeutic activity is the leave. This study was aimed to isolate the marker compound of kelor
leaves other than quersetin. The study began with preparation, characterization, and
phytochemical screening of crude drugs. Kelor leaves crude drugs were extracted by maceration
method, using ethanol. The ethanol extract was fractionated by liquid-liquid extraction using nhexane and ethyl acetate as solvent. Ethyl acetat fraction was sub-fractionated by vacuum liquid
chromatography (VLC). The selected sub-fraction from VLC was then sub-fractionated II by
classical coloumn chromatography. The isolate was purified by preparative thin layer
chromatography and purity testing was done using a single development thin layer
chromatography and two-dimensional TLC. The isolate was characterized using TLC with specific
visualization reagent and UV-Vis spectrophometric instuments to determine the class of
compounds and structure. The purification resulted a pure crystalline compound. Isolated
compound was assumed to be a group of phenolic acid or flavonoid. Isolate showed maximum
wavelength of ???nm, ???nm, ???nm and gave blue fluorosence under UV ????nm; Moringa
leaves crude drugs contained flavonoid and steroid/triterpenoid groups. Isolate K was suspected
to be flavonoid compounds which had free OH at C7, without free OH at C3 and C5 |
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