ISOLASI SENYAWA IDENTITAS DARI DAUN KELOR (MORINGA OLEIFERALAMK., MORINGACEAE)

• Main Author: Ulfa Anisah, Nur
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/44368
• Institution: Institut Teknologi Bandung

Marker compounds are specific compounds present in plant with a high amount or are the dominan compound. The marker compound may be used as a reference in the determination of the content of an extract or used in the confirmation identity of a plant and standardization for herbal medicine. Moringa oleifera Lamk is one of the plants which used by community with many pharmacological activities. Plant part of moringa plants that are often used and has therapeutic activity is the leave. This study was aimed to isolate the marker compound of kelor leaves other than quersetin. The study began with preparation, characterization, and phytochemical screening of crude drugs. Kelor leaves crude drugs were extracted by maceration method, using ethanol. The ethanol extract was fractionated by liquid-liquid extraction using nhexane and ethyl acetate as solvent. Ethyl acetat fraction was sub-fractionated by vacuum liquid chromatography (VLC). The selected sub-fraction from VLC was then sub-fractionated II by classical coloumn chromatography. The isolate was purified by preparative thin layer chromatography and purity testing was done using a single development thin layer chromatography and two-dimensional TLC. The isolate was characterized using TLC with specific visualization reagent and UV-Vis spectrophometric instuments to determine the class of compounds and structure. The purification resulted a pure crystalline compound. Isolated compound was assumed to be a group of phenolic acid or flavonoid. Isolate showed maximum wavelength of ???nm, ???nm, ???nm and gave blue fluorosence under UV ????nm; Moringa leaves crude drugs contained flavonoid and steroid/triterpenoid groups. Isolate K was suspected to be flavonoid compounds which had free OH at C7, without free OH at C3 and C5