FORMULATION AND EVALUATION OF SOLID LIPID NANOPARTICLES CAFFEINE GEL AS AN ANTI- CELLULITE

• Main Author: Syarifah, Arini
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/44434
• Institution: Institut Teknologi Bandung

Caffeine is an alkaloid which could be used as an anticellulite by inducing lipolysis through inhibition of phosphodiestherase enzyme and increase adenosine phosphate (AMP) cycles in adipose cells. Caffeine has a Log P value (octanol/water) -0,07, therefore as the consequence, caffeine will have a difficulty to penetrate through the stratum corneum. One strategy to deliver lipophobic active ingredient for topical application is solid lipid nanoparticle (SLN). In this current study, SLN was formed using double emulsion method with composition of 6% glyceryl palmitostearic, 3% poloxamer 407, 0,3% Span 20, and 0,6% caffeine. Characterization of SLN was performed, incuding particle size determination, polidispersity index, entrapment efficiency, morphology, and physical stability. The particle size of SLN were 112,60±2,65; polydisersity index 0,25±0,04; and entrapment efficiency 63.36±4,14%. Analysis using Transmission Electron Microscope (TEM) showed that SLN formed was spherical and physically stable at room temperature and 40°C for 30 days. Caffeine SLN was then formulated into a gel using HPMC. Tocopherol and isopropyl miristate as a penetration enhancer. Efficacy of caffeine SLN gel was evaluated using histopathology by analyzing adipose tissue following application of SLN caffeine gel and commercial caffeine gel for 21 days in rats. Results showed that reduction of adipose tissue layer of 20,30% and 37,19% after application of caffeine SLN gel and commercial caffeine gel, respectively. The reduction was statistically significant (p < 0.05).